Estimation of incidence of respiratory syncytial virus infection in schoolchildren using salivary antibodies

An assay for respiratory syncytial virus (RSV)–specific IgG in saliva is described. The assay was used to examine the incidence of RSV infection in schoolchildren 7–10 years old during one RSV season. One hundred and twenty‐one volunteer children provided saliva samples in October 1997 and March 199...

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Bibliographic Details
Published in:Journal of medical virology 2000-05, Vol.61 (1), p.81-84
Main Authors: Wilson, Steven D., Roberts, Kathryn, Hammond, Karen, Ayres, Jon G., Cane, Patricia A.
Format: Article
Language:English
Subjects:
Antibodies, Viral - analysis
antibody levels
Biological and medical sciences
Child
Enzyme-Linked Immunosorbent Assay
Epidemiology
Female
Fundamental and applied biological sciences. Psychology
Human viral diseases
Humans
Immunoglobulin G - analysis
Infectious diseases
Male
Medical sciences
Microbiology
respiratory syncytial virus
Respiratory Syncytial Virus Infections - epidemiology
Respiratory Syncytial Virus Infections - virology
Respiratory Syncytial Virus, Human - immunology
saliva
Salivary Glands - virology
Viral diseases
Viral diseases of the respiratory system and ent viral diseases
Virology
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Summary:An assay for respiratory syncytial virus (RSV)–specific IgG in saliva is described. The assay was used to examine the incidence of RSV infection in schoolchildren 7–10 years old during one RSV season. One hundred and twenty‐one volunteer children provided saliva samples in October 1997 and March 1998; 18% of the children showed a fourfold or greater rise in anti‐RSV IgG in the second sample. This prevalence of antibody increase is similar to that described in previous studies that measured CFT levels in serum samples. Overall, the children who showed rises in antibody levels, indicating that they had experienced an RSV infection, had lower levels of RSV‐specific antibody in their preseason samples than those who showed no increase (P = 0.0018). These results show that saliva is an adequate substitute for serum in some antibody tests and may be useful for community studies. Such studies may provide surrogate markers for susceptibility to infection, which should benefit the planning of vaccination strategies. J. Med. Virol. 61:81–84, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:0146-6615
1096-9071
DOI:10.1002/(SICI)1096-9071(200005)61:1<81::AID-JMV13>3.0.CO;2-O