High prevalence of renal transplant recipients infected with more than one cytomegalovirus glycoprotein B genotype

• Published in: Journal of medical virology 2000-05, Vol.61 (1), p.138-142
• Main Authors: Aquino, Víctor Hugo, Figueiredo, Luiz Tadeu Moraes
• Format: Article
• Language: English
• Subjects: Biological and medical sciences; cytomegalovirus; Cytomegalovirus - genetics; Cytomegalovirus Infections - epidemiology; Cytomegalovirus Infections - virology; DNA, Viral - analysis; Epidemiology; Female; Fundamental and applied biological sciences. Psychology; Genotype; glycoprotein B; Human viral diseases; Humans; Infectious diseases; Kidney Transplantation - adverse effects; Male; Medical sciences; Microbiology; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Prevalence; Prospective Studies; renal transplantation; Sensitivity and Specificity; Viral diseases; Viral diseases of the lymphoid tissue and the blood. Aids; Viral Envelope Proteins - blood; Viral Envelope Proteins - genetics; Viral Envelope Proteins - urine; Virology
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/(SICI)1096-9071(200005)61:1<138::AID-JMV22>3.0.CO;2-#

A prospective analysis of cytomegalovirus (CMV) glycoprotein B (gB) genotypes was conducted on 34 renal transplant recipients using peripheral blood leukocytes (PBLs) and urine specimens. The CMV gB genotypes were analyzed by polymerase chain reaction (PCR) followed by enzyme digestion. PBLs and urine samples showed almost equal proportions of the 4 known gB genotypes, as well as equal proportions of gB genotype mixtures. The gB genotypes 1, 2 and 3 were equally distributed in the patients. Twenty‐four (70.6%) patients had more than one gB genotype during follow‐up. There was no association of gB genotypes with the development of symptomatic CMV infection. J. Med. Virol. 61:138–142, 2000. © 2000 Wiley‐Liss, Inc.