Role of adhesion molecules in recruitment of Vdelta1 T cells from the peripheral blood to the tumor tissue of esophageal cancer patients

The mechanism responsible for tissue specific localization of gammadelta T cell subsets is not well understood. In order to explain the sequestration of specific gammadelta T cell subsets in the peripheral blood and tumor tissue of patients with esophageal cancer, we examined the function and expres...

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Bibliographic Details
Published in:Cancer immunology, immunotherapy : CII immunotherapy : CII, 2001-06, Vol.50 (4), p.218-225
Main Authors: Thomas, M L, Badwe, R A, Deshpande, R K, Samant, U C, Chiplunkar, S V
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal - immunology
Carcinoma, Squamous Cell - immunology
Cell Adhesion
Cell Adhesion Molecules - physiology
Esophageal Neoplasms - immunology
Fibroblasts - immunology
Flow Cytometry
Humans
Lymphocyte Activation
Lymphocytes, Tumor-Infiltrating - immunology
Receptors, Antigen, T-Cell, alpha-beta - immunology
Receptors, Antigen, T-Cell, gamma-delta - immunology
Tumor Cells, Cultured
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Summary:The mechanism responsible for tissue specific localization of gammadelta T cell subsets is not well understood. In order to explain the sequestration of specific gammadelta T cell subsets in the peripheral blood and tumor tissue of patients with esophageal cancer, we examined the function and expression of adhesion molecules on these cells. A hierarchy in the expression of adhesion molecules was observed. In vitro activated gammadelta T cells showed dominant expression of LFA-1 (CD11a), VLA-alpha4 (CD49d), intermediate expression of VLA-alpha5 (CD49e) and L-selectin (CD62L), but low expression of CD44v6 and alphaEbeta7 (CD103). It was observed that the gammadelta T cells use LFA-1, L-selectin and CD44v6 to bind to squamous cell carcinoma (SCC) cells, whereas they adhere to fibroblast cells using LFA-1, VLA-alpha4 and VLA-alpha5. Vdelta1 T cell subsets from the peripheral blood gammadelta T cells utilize a larger array of adhesion molecules, namely LFA-1, VLA-alpha4, VLA-alpha5, L-selectin and alphaEbeta7, to bind to SCC cells compared to the restricted usage of LFA-1, L-selectin and CD44v6 by the Vdelta2 T cells. Flow cytometric analysis of tumor infiltrating lymphocytes from the esophageal tumors confirmed the selective accumulation of Vdelta1+ gammadelta T cells in the tumor compartment. It thus appears that adhesion molecules expressed on these lymphocytes play an important role in the recruitment and retention of Vdelta1 T cells in the tumor milieu.
ISSN:0340-7004