Calreticulin Affects β-Catenin-associated Pathways

Calreticulin, a Ca2+ storage protein and chaperone in the endoplasmic reticulum, also modulates cell adhesiveness. Overexpression of calreticulin correlates with (i) increased cell adhesiveness, (ii) increased expression of N-cadherin and vinculin, and (iii) decreased protein phosphorylation on tyro...

Full description

Saved in:
Bibliographic Details
Published in:The Journal of biological chemistry 2001-07, Vol.276 (29), p.27083-27089
Main Authors: Fadel, Marc P., Szewczenko-Pawlikowski, Malgorzata, Leclerc, Pierre, Dziak, Ewa, Symonds, J. Matthew, Blaschuk, Orest, Michalak, Marek, Opas, Michal
Format: Article
Language:English
Subjects:
Animals
Base Sequence
beta Catenin
Calcium-Binding Proteins - physiology
Calreticulin
catenin
Cell Adhesion
Cell Line
Cytoskeletal Proteins - chemistry
Cytoskeletal Proteins - metabolism
DNA Primers
Endoplasmic Reticulum - enzymology
Endoplasmic Reticulum - metabolism
Fibroblasts - metabolism
Mice
Phosphorylation
Protein Tyrosine Phosphatases - metabolism
Protein-Tyrosine Kinases - metabolism
Reverse Transcriptase Polymerase Chain Reaction
Ribonucleoproteins - physiology
Signal Transduction
Trans-Activators
Tyrosine - metabolism
Wnt protein
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Calreticulin, a Ca2+ storage protein and chaperone in the endoplasmic reticulum, also modulates cell adhesiveness. Overexpression of calreticulin correlates with (i) increased cell adhesiveness, (ii) increased expression of N-cadherin and vinculin, and (iii) decreased protein phosphorylation on tyrosine. Among proteins that are dephosphorylated in cells that overexpress calreticulin is β-catenin, a structural component of cadherin-dependent adhesion complexes, a member of the armadillo family of proteins and a part of the Wnt signaling pathway. We postulate that the changes in cell adhesiveness may be due to calreticulin-mediated effects on a signaling pathway from the endoplasmic reticulum, which impinges on the Wnt signaling pathwayvia the cadherin/catenin protein system and involves changes in the activity of protein-tyrosine kinases and/or phosphatases.
ISSN:0021-9258
1083-351X
DOI:10.1074/jbc.M101676200