Antioxidant Activity of Fraxetin: In Vivo and ex Vivo Parameters in Normal Situation versus Induced Stress

The aim of this paper was to study the influence of Fraxetin (7,8-dihydroxy-6-methoxy coumarin) treatment in a Drosophila melanogaster experimental model, analyzing several parameters in normal situations and instances of induced oxidative stress. Fraxetin treatment was introduced at different ages....

Full description

Saved in:
Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2001, Vol.24(7), pp.777-784
Main Authors: FERNÁNDEZ-PUNTERO, Belén, BARROSO, Irene, IGLESIAS, Irene, BENEDÍ, Juana, VILLAR, Angel
Format: Article
Language:English
Subjects:
aging
Animals
Antioxidants - pharmacology
Biological and medical sciences
Coumarins - pharmacology
Drosophila melanogaster
Female
Fraxetin
free radicals
General and cellular metabolism. Vitamins
Glutathione - metabolism
life span
Lipid Peroxidation - drug effects
longevity
Male
Malondialdehyde - metabolism
Medical sciences
Oxidative Stress - drug effects
Pharmacology. Drug treatments
Sex Characteristics
Space life sciences
Survival Analysis
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The aim of this paper was to study the influence of Fraxetin (7,8-dihydroxy-6-methoxy coumarin) treatment in a Drosophila melanogaster experimental model, analyzing several parameters in normal situations and instances of induced oxidative stress. Fraxetin treatment was introduced at different ages. Antigravity capacity and survival parameters were evaluated as in vivo assays, and levels of oxidative status, glutathione and lipid peroxidation, as ex vivo assays. The stress situation was induced by negative geotaxis, so physical exercise enhanced its basal metabolism, generating free radicals, which are probably implicated in the molecular damage related to the aging process. In our study, all treatment groups demonstrated a beneficial effect on the evaluated parameters. So, in vivo Fraxetin protects fruit flies against oxidative stress and improves the survival parameters. Moreover, Fraxetin prevents oxidative stress by an important increase in antioxidant reserves of GSH, and peroxidative damage is preserved by Fraxetin treatments.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.24.777