Coordinate expression of secretory phospholipase A(2) and cyclooxygenase-2 in activated human keratinocytes

PGE(2) levels are altered in human epidermis after in vivo wounding; however, mechanisms modulating PGE(2) production in activated keratinocytes are unclear. In previous studies, we showed that PGE(2) is a growth-promoting autacoid in human primary keratinocyte cultures, and its production is modula...

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Published in:American Journal of Physiology: Cell Physiology 2000-04, Vol.278 (4), p.C822-C833
Main Authors: Rys-Sikora, K E, Konger, R L, Schoggins, J W, Malaviya, R, Pentland, A P
Format: Article
Language:English
Subjects:
Cells, Cultured
Cyclooxygenase 2
Dinoprostone - metabolism
Fatty Acids - metabolism
Humans
Isoenzymes - metabolism
Keratinocytes - enzymology
Keratinocytes - metabolism
Keratinocytes - pathology
Keratinocytes - physiology
Membrane Proteins
Phospholipases A - secretion
Prostaglandin-Endoperoxide Synthases - metabolism
Receptors, Cell Surface - metabolism
Receptors, Phospholipase A2
Reverse Transcriptase Polymerase Chain Reaction
Wound Healing - physiology
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Summary:PGE(2) levels are altered in human epidermis after in vivo wounding; however, mechanisms modulating PGE(2) production in activated keratinocytes are unclear. In previous studies, we showed that PGE(2) is a growth-promoting autacoid in human primary keratinocyte cultures, and its production is modulated by plating density, suggesting that regulated PGE(2) synthesis is an important component of wound healing. Here, we examine the role of phospholipase A(2) (PLA(2)) and cyclooxygenase (COX) enzymes in modulation of PGE(2) production. We report that the increased PGE(2) production that occurs in keratinocytes grown in nonconfluent conditions is also observed after in vitro wounding, indicating that similar mechanisms are involved. This increase was associated with coordinate upregulation of both COX-2 and secretory PLA(2) (sPLA(2)) proteins. Increased sPLA(2) activity was also observed. By RT-PCR, we identified the presence of type IIA and type V sPLA(2), along with the M-type sPLA(2) receptor. Thus the coordinate expression of sPLA(2) and COX-2 may be responsible for the increased prostaglandin synthesis in activated keratinocytes during wound repair.
ISSN:0363-6143
DOI:10.1152/ajpcell.2000.278.4.C822