Preparation of oligonucleotides without aldehyde abasic sites

High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites...

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Bibliographic Details
Published in:Bioorganic & medicinal chemistry letters 2001-07, Vol.11 (14), p.1863-1867
Main Authors: KROTZ, Achim H, GAUS, Hans, RAVIKUMAR, Vasulinga T, COLE, Douglas L
Format: Article
Language:English
Subjects:
Aldehydes - chemistry
Analytical, structural and metabolic biochemistry
Base Sequence
Biological and medical sciences
Chromatography, High Pressure Liquid
Fundamental and applied biological sciences. Psychology
Hydroxylamine - chemistry
Nucleic acids
Nucleic bases, nucleotides
Oligonucleotides - chemistry
Oligonucleotides - isolation & purification
Purines - chemistry
Sequence Deletion - physiology
Spectrometry, Mass, Electrospray Ionization
Surface-Active Agents - chemistry
Water - chemistry
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Summary:High-quality oligonucleotides are obtained by selective modification of sequences containing aldehyde apurinic sites with a new chromatographic tag followed by RP-HPLC separation. Hydroxylamine derivative 1 of a water soluble nonionic surfactant modifies oligonucleotides selectively at abasic sites leading to significantly increased retention.
ISSN:0960-894X
1464-3405
DOI:10.1016/S0960-894X(01)00307-9