Upregulation of phospholipase D in astrocytes in response to transient forebrain ischemia

Previous in vitro studies using cell cultures or brain slices have demonstrated that phospholipase D (PLD) in the nervous system is involved in the signaling mechanism in response to a variety of agonists. However, little is known about the pathophysiological role of PLD‐mediated signaling in the ad...

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Published in:Glia 2000-05, Vol.30 (3), p.311-317
Main Authors: Lee, Mun-Yong, Kim, Seong Yun, Min, Do Sik, Choi, Yun-Sik, Shin, Soon-Lim, Chun, Myung-Hoon, Lee, Sang Bok, Kim, Myung-Suk, Jo, Yang-Hyeok
Format: Article
Language:English
Subjects:
Animals
Astrocytes - metabolism
Astrocytes - physiology
Blotting, Western
Brain Ischemia - metabolism
Brain Ischemia - physiopathology
cerebral ischemia
GFAP
hippocampus
Hippocampus - metabolism
Hippocampus - physiopathology
Immunohistochemistry
Male
Phospholipase D - metabolism
phospholipase D1
Prosencephalon - metabolism
Prosencephalon - physiopathology
Rats
Rats, Sprague-Dawley
reactive astrocytes
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Summary:Previous in vitro studies using cell cultures or brain slices have demonstrated that phospholipase D (PLD) in the nervous system is involved in the signaling mechanism in response to a variety of agonists. However, little is known about the pathophysiological role of PLD‐mediated signaling in the adult brain. We examined the changes in the expression of a PLD isozyme, PLD1, in the adult rat hippocampus, using immunological approaches and an assay for PLD activity after transient forebrain ischemia (four‐vessel occlusion model) that results in the selective delayed death of CA1 pyramidal cells and induces reactive astrocytes in the CA1 subfield. In the control hippocampus, PLD1 the level of immunoreactivity was very low. After ischemia, in parallel with the results of Western blot analysis and the PLD activity assay, immunohistochemical analysis of PLD1 demonstrated that the immunoreactive proteins peaked at 7–14 days and were most prominent in the CA1 and the dentate hilar region. The temporal and spatial patterns of immunoreactivity of both PLD1 and glial fibrillary acidic protein (GFAP) were very similar, indicating that reactive astrocytes express PLD1, confirmed by double staining for PLD1 and GFAP. These results demonstrate that reactive astrocytes upregulate PLD in vivo after injury in the adult rat hippocampus. GLIA 30:311–317, 2000. © 2000 Wiley‐Liss, Inc.
ISSN:0894-1491
1098-1136
DOI:10.1002/(SICI)1098-1136(200005)30:3<311::AID-GLIA10>3.0.CO;2-K