Production of reactive oxygen species by spermatozoa undergoing cooling, freezing, and thawing

In the present study, we provide evidence for the production of reactive oxygen species (ROS) during cryopreservation of bovine spermatozoa. Cooling and thawing of spermatozoa cause an increase in the generation of superoxide radicals. Although nitric oxide production remains unaltered during sperm...

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Bibliographic Details
Published in:Molecular reproduction and development 2001-08, Vol.59 (4), p.451-458
Main Authors: Chatterjee, Suvro, Gagnon, Claude
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cell physiology
Cryopreservation
Cryoprotective Agents - chemistry
Cryoprotective Agents - metabolism
Effects of physical and chemical agents
Electron Spin Resonance Spectroscopy
Fundamental and applied biological sciences. Psychology
Hydrogen Peroxide - metabolism
lipid peroxidation
Male
membrane fluidity
Membrane Fluidity - physiology
Molecular and cellular biology
nitric oxide
Nitric Oxide - metabolism
Reactive Oxygen Species - metabolism
Spermatozoa - physiology
Spin Trapping
superoxide
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Summary:In the present study, we provide evidence for the production of reactive oxygen species (ROS) during cryopreservation of bovine spermatozoa. Cooling and thawing of spermatozoa cause an increase in the generation of superoxide radicals. Although nitric oxide production remains unaltered during sperm cooling from 22–4°C, a sudden burst of nitric oxide radicals is observed during thawing. Increase in lipid peroxidation levels have been observed in frozen/thawed spermatozoa and appears to be associated with a reduction in sperm membrane fluidity as detected by spin labeling studies. The data presented provide strong evidence that oxygen free radicals are produced during freezing and thawing of bovine spermatozoa and suggest that these reactive oxygen species may be a cause for the decrease in sperm function following cryopreservation. Mol. Reprod. Dev. 59: 451–458, 2001. © 2001 Wiley‐Liss, Inc.
ISSN:1040-452X
1098-2795
DOI:10.1002/mrd.1052