Effect of triiodothyronine on phospholipid metabolism in skeletal muscles of the rat

The aim of the present study was to examine the effect of treatment with triiodothyronine (T3) on certain aspects of phospholipid metabolism in skeletal muscles. Rats were injected with triiodothyronine (T3) daily (10 microg x 100 g(-1) b.w., s.c.) for six days. Saline-treated rats served as control...

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Bibliographic Details
Published in:Journal of physiology and pharmacology : an official journal of the Polish Physiological Society 2000-03, Vol.51 (1), p.103-110
Main Authors: Zendzian-Piotrowska, M, Górska, M, Dworakowski, W, Górski, J
Format: Article
Language:English
Subjects:
Animals
Dose-Response Relationship, Drug
Male
Muscle, Skeletal - drug effects
Muscle, Skeletal - metabolism
Palmitic Acid - blood
Palmitic Acid - metabolism
Phosphatidylinositols - metabolism
Phosphatidylserines - metabolism
Phospholipids - metabolism
Rats
Rats, Wistar
Triiodothyronine - pharmacology
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Summary:The aim of the present study was to examine the effect of treatment with triiodothyronine (T3) on certain aspects of phospholipid metabolism in skeletal muscles. Rats were injected with triiodothyronine (T3) daily (10 microg x 100 g(-1) b.w., s.c.) for six days. Saline-treated rats served as controls. 24 h after the last dose of T3, 14C palmitic acid suspended in the serum of a donor rat, was administered intravenously. Thirty min later, samples of the soleus, white and red section of the gastrocnemius and blood from the abdominal aorta were taken. The muscle phospholipids were extracted and separated into different fractions by means of thin layer chromatography. The following fractions were obtained: shingomeylin, phosphatidylcholine phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and cardiolipin. The phospholipids were quantified and their radioactivity was measured. The plasma free fatty acid concentration and radioactivity was also determined. Treatment with T3 reduced the content of phosphatidylinositol and phosphatidylserine in each muscle type, whereas the concentration of other phospholipids remained stable. T3 increased markedly incorporation of the blood-borne fatty acids into each phospholipid fraction in the muscles. It is concluded that an excess of T3 influences the metabolism of phospholipids in skeletal muscles.
ISSN:0867-5910