Localisation and characterisation of ovine immunglobulin within the sheep scab mite, Psoroptes ovis

Ovine IgG was detected in homogenates of repeatedly washed Psoroptes ovis. Some of the immunoglobulin in the homogenates was fragmented although the host IgG present in mite washings was largely intact. The host immunoglobulin was immuno-localised to the surface or cytoplasm of the gut cells of feed...

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Bibliographic Details
Published in:Veterinary parasitology 2000-04, Vol.89 (3), p.231-239
Main Authors: Pettit, D., Smith, W.D., Richardson, J., Munn, E.A.
Format: Article
Language:English
Subjects:
Animals
digestion
digestive tract
Electrophoresis, Polyacrylamide Gel - veterinary
immunocytochemistry
immunoglobulin G
Immunoglobulins
Immunoglobulins - analysis
Immunohistochemistry
immunolocalization
Ingestion
localization
Mite Infestations - immunology
Mite Infestations - parasitology
Mite Infestations - veterinary
Mites - immunology
Psoroptes cuniculi
Psoroptes ovis
Rabbits
Sheep
Sheep Diseases - immunology
Sheep Diseases - parasitology
Sheep-Arthropoda
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Summary:Ovine IgG was detected in homogenates of repeatedly washed Psoroptes ovis. Some of the immunoglobulin in the homogenates was fragmented although the host IgG present in mite washings was largely intact. The host immunoglobulin was immuno-localised to the surface or cytoplasm of the gut cells of feeding stages of freshly harvested P. ovis examined by cryosectioning. A similar distribution of rabbit IgG was detected in P. cuniculi. The IgG demonstrated in the mite gut represented partially digested as well as intact immunoglobulin. The presence of intact host immunoglobulin suggests that P. ovis may be susceptible to vaccination by the gut antigen approach, a method used successfully for blood-feeding ectoparasites like Boophilus microplus.
ISSN:0304-4017
1873-2550
DOI:10.1016/S0304-4017(00)00203-X