A Brevibacterium lactofermentum 16S rRNA gene used as target site for homologous recombination

Abstract Genes for rRNA are highly conserved and present in multiple copies in most prokaryotic organisms increasing the number of theoretical sites for homologous recombination. They might be targets for integration events between unrelated microorganisms providing that an efficient genetic transfe...

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Bibliographic Details
Published in:FEMS microbiology letters 2000-04, Vol.185 (2), p.199-204
Main Authors: Amador, Eladio, Martín, Juan Francisco, Castro, José María
Format: Article
Language:English
Subjects:
16S rRNA
Brevibacterium
Brevibacterium - genetics
Brevibacterium - growth & development
Brevibacterium lactofermentum
Deactivation
Deoxyribonucleic acid
DNA
Electroporation
Gene mapping
Gene Targeting
Gene transfer
Genes, Bacterial
Genes, rRNA
Homologous recombination
Homology
Inactivation
Integration
Mapping
Microbiology
Microorganisms
Nucleic Acid Hybridization
Operons
Plasmids - genetics
Recombination, Genetic
RNA, Ribosomal, 16S - genetics
rRNA 16S
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Summary:Abstract Genes for rRNA are highly conserved and present in multiple copies in most prokaryotic organisms increasing the number of theoretical sites for homologous recombination. They might be targets for integration events between unrelated microorganisms providing that an efficient genetic transfer is present. We have used a plasmid containing a portion of the 16S rRNA gene from the rrnD operon of Brevibacterium lactofermentum to transform the same strain resulting in non-essential inactivation of various rrn operons. Integration of the transforming DNA occurs in all cases. The system may be used to test possible gene transfer at least among closely related strains and is of great interest for integration of foreign DNA and for mapping.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09062.x