Analysis of chimaerism in thalassaemic children undergoing stem cell transplantation

We have prospectively assessed the relative contribution of host and donor to haemopoiesis following stem cell transplantation (SCT) in children with β‐thalassaemia major (n = 35), using karyotype analysis or Southern blot/polymerase chain reaction analysis of variable number tandem repeats on genom...

Full description

Saved in:
Bibliographic Details
Published in:British journal of haematology 2001-07, Vol.114 (1), p.219-225
Main Authors: Amrolia, P. J., Vulliamy, T., Vassiliou, G ., Lawson, S., Bryon, J., Kaeda, J., Dokal, I., Johnston, R., Veys, P., Darbyshire, P., Roberts, I. A. G.
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Adolescent
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
beta-Thalassemia - genetics
beta-Thalassemia - therapy
Biological and medical sciences
bone marrow transplantation
Bone marrow, stem cells transplantation. Graft versus host reaction
Child
Child, Preschool
chimaerism
Chimera
Graft Rejection - genetics
Graft vs Host Disease - genetics
Hematology
Hematopoietic Stem Cell Transplantation
Humans
Medical sciences
Minisatellite Repeats
non‐myeloablative conditioning
Prospective Studies
rejection
thalassaemia
Transfusions. Complications. Transfusion reactions. Cell and gene therapy
Transplantation Conditioning
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We have prospectively assessed the relative contribution of host and donor to haemopoiesis following stem cell transplantation (SCT) in children with β‐thalassaemia major (n = 35), using karyotype analysis or Southern blot/polymerase chain reaction analysis of variable number tandem repeats on genomic DNA from peripheral blood. Early haemopoiesis was fully donor in origin in 24 out of 35 cases and remained so throughout the post‐transplant course in all but one patient, who evolved to stable mixed chimaerism. The remaining 11 cases (31%) initially showed mixed chimaerism: four of these rejected, one eventually eradicated host haemopoiesis to become fully donor haemopoietic, and the remaining six had persistent mixed chimaerism, with 5–38% host haemopoiesis. The risk of graft rejection was high when > 15% host haemopoiesis was present at 3 months post transplant: four out of six such patients rejected their grafts; conversely, zero out of 29 patients with
ISSN:0007-1048
1365-2141
DOI:10.1046/j.1365-2141.2001.02931.x