Comparison of Tissue Stages of Hepatozoon americanum in the Dog Using Immunohistochemical and Routine Histologic Methods

American canine hepatozoonosis is caused by Hepatozoon americanum, a recently described species of apicomplexan protozoan parasite. An immunohistochemical procedure using a polyclonal antibody to sporozoites of H. americanum clearly identified asexual stages of H. americanum in canine striated muscl...

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Bibliographic Details
Published in:Veterinary pathology 2001-07, Vol.38 (4), p.422-426
Main Authors: Panciera, R. J., Mathew, J. S., Cummings, C. A., Duffy, J. C., Ewing, S. A., Kocan, A. A.
Format: Article
Language:English
Subjects:
Animals
Coccidiosis - parasitology
Coccidiosis - pathology
Coccidiosis - veterinary
Dog Diseases - parasitology
Dog Diseases - pathology
Dogs
Eucoccidiida - growth & development
Host-Parasite Interactions
Immunohistochemistry - veterinary
Muscle, Skeletal - parasitology
Muscle, Skeletal - pathology
Rabbits
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Summary:American canine hepatozoonosis is caused by Hepatozoon americanum, a recently described species of apicomplexan protozoan parasite. An immunohistochemical procedure using a polyclonal antibody to sporozoites of H. americanum clearly identified asexual stages of H. americanum in canine striated muscle. The method also detects hepatozoa present in naturally infected coyotes and raccoons and reacts with certain other apicomplexans. Use of this immunohistochemical procedure confirms the canine intermediate host-parasite relationships that were presumptively established using conventional histopathologic methods.
ISSN:0300-9858
1544-2217
DOI:10.1354/vp.38-4-422