High-performance liquid chromatographic analysis of mometasone furoate and its degradation products: Application to in vitro degradation studies

A method of analysis of mometasone furoate in pharmaceutical formulations and biological fluids is necessary to study the degradation kinetics and determine its stability. A simple high-performance liquid chromatographic method was developed for simultaneous determination of mometasone furoate and i...

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Bibliographic Details
Published in:Journal of pharmaceutical and biomedical analysis 2001-09, Vol.26 (2), p.313-319
Main Authors: Teng, Xiao Wei, Foe, Kuncoro, Brown, Kenneth F, Cutler, David J, Davies, Neal M
Format: Article
Language:English
Subjects:
Administration, Topical
Analysis
Anti-Inflammatory Agents - analysis
Anti-Inflammatory Agents - blood
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Drug Stability
General pharmacology
Glucocorticoids
Human plasma
Humans
Kinetics
Medical sciences
Mometasone Furoate
Pharmacology. Drug treatments
Pregnadienediols - analysis
Pregnadienediols - blood
Reversed-phase HPLC
Spectrophotometry, Ultraviolet - methods
UV-detection
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Summary:A method of analysis of mometasone furoate in pharmaceutical formulations and biological fluids is necessary to study the degradation kinetics and determine its stability. A simple high-performance liquid chromatographic method was developed for simultaneous determination of mometasone furoate and its degradation products in human plasma. Plasma (0.5 ml) was extracted with dichloromethane after addition of the internal standard, dexamethasone 21-acetate. Separation was achieved on a Beckman C 8 column with UV detection at 248 nm. The calibration curve was linear ranging from 0.2 to 100 μg/ml. The mean extraction efficiency was >86%. Precision of the assay was
ISSN:0731-7085
1873-264X
DOI:10.1016/S0731-7085(01)00408-3