Dendritic hypertrophy of Stach type VI neurons within experimentally altered ileum of pigs

• Published in: Autonomic neuroscience 2001-06, Vol.89 (1), p.31-37
• Main Authors: Brehmer, Axel, Frieser, Markus, Graf, Michael, Radespiel-Tröger, Martin, Göbel, Dieter, Neuhuber, Winfried
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Cell Size - physiology; Dendrites - physiology; Enteric nervous system; Fundamental and applied biological sciences. Psychology; Gut; Hypertrophy; Ileum - innervation; Ileum - pathology; Ileum - surgery; Innervation; Intestine. Mesentery; Male; Morphology; Muscle, Smooth - innervation; Muscle, Smooth - pathology; Muscle, Smooth - surgery; Myenteric Plexus - cytology; Neurons - physiology; Neurons - ultrastructure; Peristalsis; Plasticity; Stenosis; Stress, Mechanical; Swine; Vertebrates: digestive system
• ISSN: 1566-0702; 1872-7484
• DOI: 10.1016/S1566-0702(01)00250-8

Myenteric neurons were investigated morphometrically to answer the question if type-specific somal hypertrophy of type VI neurons in mechanically stressed ileum of pigs, which was known from an earlier study, is correlated with an increased dendritic arborization, that is, with dendritic hypertrophy. Muscular hypertrophy was induced in the ileum of two juvenile pigs by narrowing the gut circumference (mechanical stenosis) and by reversing a loop of ileum which results in an antiperistaltic segment (functional stenosis), respectively. After a survival time of 6 weeks, wholemounts from the pre- and poststenotic ileal regions, from the antiperistaltic segment as well as from an age matched control animal, were silver impregnated. Dendritic parameters of Stach types IV and VI neurons were recorded using a computer-aided morphometric program and analysed statistically. Type IV neurons showed no change of dendritic parameters, neither within control nor within stenosed ileal segments. In contrast, the type VI neurons displayed increased dendritic parameters within zones of muscular hypertrophy such as total dendritic length, numbers of dendrites, of dendritic branching points and of dendritic endings. We suggest that type VI neurons may participate as descending nitrergic interneurons or motorneurons in the control of muscular function, thus, undergoing plastic changes in case of experimental muscular hypertrophy. Type IV neurons which are involved in the regulation of mucosal processes were not affected by muscular hypertrophy.