Crevicular fluid elastase levels in relation to periodontitis and metabolic control of diabetes

• Published in: Journal of periodontal research 2001-06, Vol.36 (3), p.169-174
• Main Authors: Alpagot, Tamer, Silverman, Sol, Lundergan, William, Bell, Colin, Chambers, David W.
• Format: Article
• Language: English
• Subjects: Adult; Age Factors; Associated diseases and complications; Benzoylarginine Nitroanilide; Bicuspid; Biological and medical sciences; Chromogenic Compounds; Dentistry; diabetes; Diabetes Mellitus, Type 1 - enzymology; Diabetes Mellitus, Type 1 - prevention & control; Diabetes Mellitus, Type 2 - enzymology; Diabetes Mellitus, Type 2 - prevention & control; Diabetes. Impaired glucose tolerance; elastase; Endocrine pancreas. Apud cells (diseases); Endocrinopathies; Enzyme-Linked Immunosorbent Assay; Facial bones, jaws, teeth, parodontium: diseases, semeiology; Female; gingival crevicular fluid; Gingival Crevicular Fluid - enzymology; Gingival Hemorrhage - enzymology; Glycated Hemoglobin A - analysis; Humans; Leukocyte Elastase - analysis; level of diabetic control; Linear Models; Male; Medical sciences; Middle Aged; Molar; Non tumoral diseases; Otorhinolaryngology. Stomatology; Pancreatic Elastase - analysis; Periodontal Attachment Loss - enzymology; Periodontal Index; Periodontal Pocket - enzymology; periodontitis; Periodontitis - enzymology; Regression Analysis; Risk Factors; Smoking - adverse effects; Statistics as Topic
• ISSN: 0022-3484; 1600-0765
• DOI: 10.1034/j.1600-0765.2001.360305.x

The purpose of this study was to investigate the associations between gingival crevicular fluid (GCF) elastase levels, clinical measures of periodontal status, and metabolic control of diabetes in insulin dependent (type 1) diabetes (IDDM) and non‐insulin dependent (type 2) diabetes (NIDDM) patients. Sixty patients were recruited from the Diabetes Center at the University of California in San Francisco. Thirty subjects were type 1 diabetics and 30 subjects were type 2 diabetics. Metabolic control was evaluated by glycosylated hemoglobin (HbA1c) levels. Demographic information was obtained using a structured interview with the subjects. Clinical measurements and GCF samples were taken from the mesio‐buccal surfaces of 2 premolars and 2 molars from the most diseased sextant. GCF elastase was determined by measurement of p‐Nitroanalide resulting from hydrolysis of elastase specific peptide. Crevicular fluid elastase levels were significantly correlated with gingival index, bleeding index, probing depth and attachment level in both type 1 and type 2 diabetes groups (0.01