Hyperthermia to normal human skin in vivo upregulates heat shock proteins 27, 60, 72i and 90

This study was performed to test the hypothesis that expression of heat shock proteins (HSPs) exhibits a spatially selective response within intact human skin following in vivo exposure to thermal stress. This response is believed to protect cells and tissues from further damage. Using Western blott...

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Bibliographic Details
Published in:Journal of cutaneous pathology 2000-04, Vol.27 (4), p.176-182
Main Authors: Wilson, Niall, McArdle, Anne, Guerin, Deirdre, Tasker, Helen, Wareing, Paul, Foster, Christopher S., Jackson, Malcolm J., Rhodes, Lesley E.
Format: Article
Language:English
Subjects:
Adult
Biological and medical sciences
Blotting, Western
Chaperonin 60 - metabolism
Heat-Shock Proteins - metabolism
Hot Temperature
HSP27 Heat-Shock Proteins
HSP72 Heat-Shock Proteins
HSP90 Heat-Shock Proteins - metabolism
Humans
Immunoenzyme Techniques
Injuries of the skin. Diseases of the skin due to physical agents
Male
Medical sciences
Middle Aged
Neoplasm Proteins - metabolism
Skin - metabolism
Stress, Physiological - metabolism
Traumas. Diseases due to physical agents
Up-Regulation
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Summary:This study was performed to test the hypothesis that expression of heat shock proteins (HSPs) exhibits a spatially selective response within intact human skin following in vivo exposure to thermal stress. This response is believed to protect cells and tissues from further damage. Using Western blotting and immunohistochemistry, we studied the expression of a range of HSPs in normal human skin of 5 subjects prior to and following heating in vivo. The skin was heated to 41±0.5°C for 1 h and biopsies were taken at 4, 8 and 24 h and from control, untreated skin. HSPs 27, 60, 72i, 90, 110 and heat shock constitutive (HSC)70 were expressed in normal skin, but the extent and distribution of these HSPs showed considerable variation. HSP27, 60 and 72i were found predominantly in the epidermis, whereas HSC70 showed weak epidermal staining but strong dermal expression. Heating the skin in vivo resulted in an increased skin content of HSP27, 60, 72i and 90, with maximal increase at 24 h following hyperthermia, while the skin content of HSC70 and HSP110 were unchanged. Significant increases in the content of HSP72i and HSP90 had occurred by 4 h following hyperthermia, with a mean ±SEM of 206±50% and 197±38% of the control, untreated values, respectively (p
ISSN:0303-6987
1600-0560
DOI:10.1034/j.1600-0560.2000.027004176.x