Quantitative determination of SC-68328 in dog plasma using flow injection and tandem mass spectrometry

A flow injection/tandem mass spectrometric assay was developed to quantitate SC‐68328 in dog plasma using its stable isotopic analog [13C4]SC‐68328 as an internal standard (IS). Since SC‐68328, a manganese‐based superoxide dismutase mimetic, is very unstable, very polar and adheres to silica‐based h...

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Bibliographic Details
Published in:Journal of mass spectrometry. 2000-03, Vol.35 (3), p.354-360
Main Authors: Zhang, Ji Y., Fast, Douglas M., Schoenhard, Grant L., Arora, Vinod K., Belas, Frank J., Blair, Ian A.
Format: Article
Language:English
Subjects:
Analysis
Animals
Biological and medical sciences
Blood Chemical Analysis - methods
Blood Chemical Analysis - standards
Blood Chemical Analysis - statistics & numerical data
Dogs
electrospray ionization
flow injection
General pharmacology
Mass Spectrometry - methods
Mass Spectrometry - standards
Mass Spectrometry - statistics & numerical data
Medical sciences
method development and validation
Organometallic Compounds - blood
Organometallic Compounds - chemistry
Organometallic Compounds - pharmacology
Pharmacology. Drug treatments
Quality Control
Superoxide Dismutase - pharmacology
superoxide dismutase mimetic
tandem mass spectrometry
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Summary:A flow injection/tandem mass spectrometric assay was developed to quantitate SC‐68328 in dog plasma using its stable isotopic analog [13C4]SC‐68328 as an internal standard (IS). Since SC‐68328, a manganese‐based superoxide dismutase mimetic, is very unstable, very polar and adheres to silica‐based high‐performance liquid chromatographic columns, the analyte and IS were derivatized to their bis‐isothiocyanate forms followed by a liquid–liquid extraction with methylene chloride and analyzed using positive ion electrospray mass spectrometric detection. SC‐68328 was quantitated using the peak‐height ratio of SC‐68328 to its IS using MS/MS in the multiple reaction monitoring mode. The lower limit of quantitation of the assay was 0.25 µg ml−1 SC‐68328 in dog plasma with an inter‐day precision of 11.8% and an accuracy of 113% (n = 12). Acceptable precision and accuracy were also obtained for concentrations in the calibration curve range (0.25–10 µg ml−1 SC‐68328 in dog plasma). Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:1076-5174
1096-9888
DOI:10.1002/(SICI)1096-9888(200003)35:3<354::AID-JMS942>3.0.CO;2-8