Effect of fasting on rat duodenal and jejunal microvilli

Duodenal and jejunal ultrastructural morphology and total serum alkaline phosphatase (ALP) activity were investigated in Wistar rats submitted to normal feeding, a 24 h fasting without or with access to sawdust or expanded polystyrene, and a 22 h fasting followed by a 2 h refeeding. Ultrastructural...

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Bibliographic Details
Published in:Clinical nutrition (Edinburgh, Scotland) Scotland), 2001-08, Vol.20 (4), p.325-331
Main Authors: MARTINS, M.J., HIPÓLITO-REIS, C., AZEVEDO, I.
Format: Article
Language:English
Subjects:
Alkaline Phosphatase - blood
Alkaline Phosphatase - metabolism
alkaline phosphatase activity
Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy
Animals
Biological and medical sciences
Duodenum - enzymology
Duodenum - pathology
Emergency and intensive care: metabolism and nutrition disorders. Enteral and parenteral nutrition
expanded polystyrene
Fasting
feeding
Intensive care medicine
intestinal microvilli
Intestinal Mucosa - enzymology
Intestinal Mucosa - pathology
Jejunum - enzymology
Jejunum - pathology
Male
Medical sciences
Microvilli - pathology
Microvilli - ultrastructure
Rats
Rats, Wistar
sawdust
starvation
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Summary:Duodenal and jejunal ultrastructural morphology and total serum alkaline phosphatase (ALP) activity were investigated in Wistar rats submitted to normal feeding, a 24 h fasting without or with access to sawdust or expanded polystyrene, and a 22 h fasting followed by a 2 h refeeding. Ultrastructural observation of duodenal and jejunal villous tips showed the occurrence of degenerating epithelial cells that were much more frequent in the epithelium of fasted rats. Refeeding and ingestion of sawdust or expanded polystyrene decreased the mean percentage of degenerating cells in the duodenum but not in the jejunum in comparison to plain fasted animals. Normal feeding produced the highest value of serum ALP activity. Refeeding as well as ingestion of sawdust or expanded polystyrene increased serum ALP activity values from those of plain fasted state: the difference being significant only for the refed group. It is concluded that: a) feeding, for both duodenum and jejunum, and refeeding and mechanical stimulation, just for the duodenum, increased proliferation, decreased apoptosis or facilitated desquamation of the epithelium; b) ALP increase in serum was induced by feeding and refeeding.
ISSN:0261-5614
1532-1983
DOI:10.1054/clnu.2001.0459