A new densovirus isolated from the mosquito Culex pipiens (Diptera: Culicidae)

We have isolated from a laboratory strain of Culex pipiens in which abnormal larval mortality occurred a small icosahedral nonenveloped DNA virus sharing the main biological and biophysical properties of densoviruses (DNVs). Unlike DNVs isolated previously from Aedes species, i.e. the AaeDNV and the...

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Bibliographic Details
Published in:Virus research 2000-03, Vol.67 (1), p.11-16
Main Authors: Jousset, Françoise-Xavière, Baquerizo, Elizabeth, Bergoin, Max
Format: Article
Language:English
Subjects:
Aedes
Animals
Capsid - analysis
Culex - anatomy & histology
Culex - virology
Culex pipiens
Culicidae
Densovirus
Densovirus - chemistry
Densovirus - genetics
Densovirus - isolation & purification
DNA, Viral - analysis
Electrophoresis, Polyacrylamide Gel
Immunoblotting
Junonia coenia
Larva
Microscopy, Fluorescence
Mosquito
Parvovirus
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Description
Summary:We have isolated from a laboratory strain of Culex pipiens in which abnormal larval mortality occurred a small icosahedral nonenveloped DNA virus sharing the main biological and biophysical properties of densoviruses (DNVs). Unlike DNVs isolated previously from Aedes species, i.e. the AaeDNV and the AalDNV (Afanasiev, B.N., Galyov, E.E., Buchatsky, L.P., Kozlov, Y.V., 1991. Nucleotide sequence and genomic organization of Aedes densonucleosis virus. Virology 185, 323–336; Boublik, Y., Jousset, F.-X., Bergoin, M., 1994. Complete nucleotide sequence and genomic organization of the Aedes albopictus Parvovirus ( AaPV) pathogenic for Aedes aegypti larvae. Virology 200, 752–763), this mosquito DNV named CpDNV possesses a genome of 6 kb separately encapsidated in stoichiometric proportion as ‘plus’ and ‘minus’ strands. The lack of sequence homology between the CpDNV and the AalDNV genome and of antigenic cross-reactivity between their capsid polypeptides indicates that these two mosquito DNVs are phylogenetically distant. In contrast, the CpDNV appears to be related to the Junonia coenia densovirus ( JcDNV) at both serological and genomic levels.
ISSN:0168-1702
1872-7492
DOI:10.1016/S0168-1702(00)00128-3