Effects of trimethoprim and co-trimoxazole on the morphology of Listeria monocytogenes in culture medium and after phagocytosis

The purpose of this study was to compare the extra- and intracellular activity of antifolates on Listeria monocytogenes. The fortuitous discovery of elongated bacteria in response to trimethoprim revealed a novel effect on the morphology of Listeria in cell culture medium and after phagocytosis. Thi...

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Bibliographic Details
Published in:Journal of antimicrobial chemotherapy 2001-08, Vol.48 (2), p.185-193
Main Authors: Minkowski, Patricia, Staege, Hannah, Groscurth, Peter, Schaffner, Andreas
Format: Article
Language:English
Subjects:
Animals
Anti-Infective Agents, Urinary - pharmacology
Antibacterial agents
Antibiotics. Antiinfectious agents. Antiparasitic agents
Biological and medical sciences
Cell Line
Cells, Cultured
Culture Media
Extracellular Space - drug effects
Extracellular Space - microbiology
Humans
Intracellular Fluid - drug effects
Intracellular Fluid - microbiology
Listeria monocytogenes - cytology
Listeria monocytogenes - drug effects
Macrophages - cytology
Macrophages - drug effects
Macrophages - microbiology
Medical sciences
Mice
Phagocytosis - drug effects
Pharmacology. Drug treatments
Trimethoprim - pharmacology
Trimethoprim, Sulfamethoxazole Drug Combination - pharmacology
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Summary:The purpose of this study was to compare the extra- and intracellular activity of antifolates on Listeria monocytogenes. The fortuitous discovery of elongated bacteria in response to trimethoprim revealed a novel effect on the morphology of Listeria in cell culture medium and after phagocytosis. This phenomenon permitted the quantification of trimethoprim activity, revealing comparable activity intra- and extracellularly. Subinhibitory concentrations of trimethoprim resulted in bacterial elongation, which was reversed after removal of trimethoprim. We attribute this effect of trimethoprim to an inhibition of cell wall synthesis and/or cell separation of Listeria.
ISSN:0305-7453
1460-2091
1460-2091
DOI:10.1093/jac/48.2.185