The centromere‐binding factor Cbf1p from Candida albicans complements the methionine auxotrophic phenotype of Saccharomyces cerevisiae

• Published in: Yeast (Chichester, England) England), 2001-08, Vol.18 (11), p.1047-1052
• Main Authors: Eck, Raimund, Stoyan, Tanja, Künkel, Waldemar
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; CaCBF1 gene; Candida albicans; Candida albicans - chemistry; Candida albicans - genetics; Cbf1 protein; Cbf1p; centromere; centromere binding factor 1; centromere-binding protein; chromosome 4; Chromosome Mapping; Chromosomes, Fungal - genetics; Cloning, Molecular; DNA, Fungal - genetics; DNA-Binding Proteins - chemistry; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; Fungal Proteins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Genes, Fungal; Genetic Complementation Test; Isoelectric Point; Methionine - metabolism; Mitosis; Molecular Sequence Data; Molecular Weight; Mutation; Phenotype; Plasmids; Promoter Regions, Genetic; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Nucleic Acid; transcription factor; Transformation, Genetic
• ISSN: 0749-503X; 1097-0061
• DOI: 10.1002/yea.757

A gene encoding the centromere binding factor 1 (Cbf1p) of the human pathogenic yeast Candida albicans was cloned and characterized. An open reading‐frame was detected which encoded a 223 amino acid protein with a calculated molecular weight of 25.8 kDa and a relative isoelectric point of 5.55. It shares 39% overall amino acid sequence identity with Saccharomyces cerevisiae Cbf1p. We localized the CaCBF1 gene on chromosome 4. Southern analysis indicated that CaCBF1 is probably present as a single copy gene per haploid genome. The CaCBF1 gene under the control of its own promoter was able to complement the methionine auxotrophic growth, the increased mitotic instability of CEN plasmids and the slow growth of a Saccharomyces cerevisiae cbf1Δ mutant strain. The GenBank Accession No. for this gene is AJ303083. Copyright © 2001 John Wiley & Sons, Ltd.