UV Resonance Raman Detection and Quantitation of Domoic Acid in Phytoplankton

Cultures of the phytoplankton diatom, Pseudonitzschia multiseries, have been harvested under controlled growth conditions ranging from late logarithmic to late stationary phase (17−58 days). The amount of domoic acid (DA) present in the growth media and in the homogenized cells has been determined b...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2000-04, Vol.72 (7), p.1666-1671
Main Authors: Wu, Q, Nelson, W. H, Treubig, J. M, Brown, P. R, Hargraves, P, Kirs, M, Feld, M, Desari, R, Manoharan, R, Hanlon, E. B
Format: Article
Language:English
Subjects:
Acids
Biological and medical sciences
Chemical constitution
Chemistry
Freshwater
Fundamental and applied biological sciences. Psychology
Kainic Acid - analogs & derivatives
Kainic Acid - analysis
Phytoplankton - chemistry
Plankton
Plant physiology and development
Pseudonitzschia multiseries
Spectrophotometry, Ultraviolet - methods
Spectrum Analysis, Raman - methods
Ultraviolet radiation
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Summary:Cultures of the phytoplankton diatom, Pseudonitzschia multiseries, have been harvested under controlled growth conditions ranging from late logarithmic to late stationary phase (17−58 days). The amount of domoic acid (DA) present in the growth media and in the homogenized cells has been determined by HPLC. Defined samples of media, homogenized cells, whole cells, and whole cells in media have been laser excited at 251 nm for the purpose of selectively exciting intense UV resonance Raman spectra from DA in the samples. Neither media nor cell component spectra from algae seriously interfere with DA spectra. The spectral cross sections for the dominant 1652-cm-1 mode of DA have been determined for 242-, 251-, and 257-nm excitation. Maximum sensitivities are achieved with 251-nm excitation because cross sections for DA are a maximum, and interference from other algal components becomes very small. DA concentrations that have been determined with 251-nm excitation by resonance Raman methods correlate closely with values determined independently with HPLC, especially at higher DA concentrations. The UV resonance Raman analysis of DA in phytoplankton algae is shown to be very sensitive and quantitative as well as rapid and nonintrusive.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac991052d