Identification of Genes Regulated During Mechanical Load-induced Cardiac Hypertrophy

Cardiac hypertrophy is associated with both adaptive and adverse changes in gene expression. To identify genes regulated by pressure overload, we performed suppressive subtractive hybridization between cDNA from the hearts of aortic-banded (7-day) and sham-operated mice. In parallel, we performed a...

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Bibliographic Details
Published in:Journal of molecular and cellular cardiology 2000-05, Vol.32 (5), p.805-815
Main Authors: Johnatty, Sharon E, Dyck, Jason R.B, Michael, Lloyd H, Olson, Eric N, Abdellatif, Maha
Format: Article
Language:English
Subjects:
Animals
Calcineurin
Calcineurin - genetics
Cardiac hypertrophy
Cardiomegaly - genetics
Gene Expression Regulation
Gene induction
Life Sciences (General)
Mice
Mice, Transgenic
Nucleic Acid Hybridization - methods
Pressure overload
Space life sciences
Subtractive hybridization
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Summary:Cardiac hypertrophy is associated with both adaptive and adverse changes in gene expression. To identify genes regulated by pressure overload, we performed suppressive subtractive hybridization between cDNA from the hearts of aortic-banded (7-day) and sham-operated mice. In parallel, we performed a subtraction between an adult and a neonatal heart, for the purpose of comparing different forms of cardiac hypertrophy. Sequencing more than 100 clones led to the identification of an array of functionally known (70%) and unknown genes (30%) that are upregulated during cardiac growth. At least nine of those genes were preferentially expressed in both the neonatal and pressure over-load hearts alike. Using Northern blot analysis to investigate whether some of the identified genes were upregulated in the load-independent calcineurin-induced cardiac hypertrophy mouse model, revealed its incomplete similarity with the former models of cardiac growth.
ISSN:0022-2828
1095-8584
DOI:10.1006/jmcc.2000.1122