The effects of various organ preservation solutions on hepatocyte membrane potentials, intracellular calcium concentrations, and outcome following liver transplantation

• Published in: The American journal of surgery 2000-02, Vol.179 (2), p.154-160
• Main Authors: Cohen, Ari J, Burczynski, Frank J, Rosser, Barry G, Lipschitz, Jeremy, Minuk, Gerald Y
• Format: Article
• Language: English
• Subjects: Abdomen; Adenosine - therapeutic use; Allopurinol - therapeutic use; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Animals; Bile ducts; Biological and medical sciences; Calcium; Calcium (intracellular); Calcium - analysis; Calcium Signaling - drug effects; Clinical death. Palliative care. Organ gift and preservation; Depolarization; Enzymes; Ethanol; Ethanol - therapeutic use; Fluorescence; Fluorescent Dyes; Fura-2; Glutathione - therapeutic use; Graft Survival - drug effects; Health risks; Hepatectomy; Hepatocytes; Hypertonic Solutions - therapeutic use; Injuries; Insulin - therapeutic use; Intracellular; Kinases; Liver; Liver - drug effects; Liver - pathology; Liver - physiopathology; Liver transplantation; Liver Transplantation - pathology; Liver Transplantation - physiology; Liver transplants; Male; Medical sciences; Membrane potential; Membrane Potentials - drug effects; Membranes; Microelectrodes; Microscopy, Fluorescence; Microscopy, Video; Organ Preservation; Organ Preservation Solutions - therapeutic use; Penis; Polyethylene; Preservation; Raffinose - therapeutic use; Rats; Rats, Sprague-Dawley; Reperfusion; Reperfusion Injury - physiopathology; Reperfusion Injury - prevention & control; Sodium Chloride; Statistics, Nonparametric; Surgery; Survival; Transplantation; Transplants; Treatment Outcome; Veins & arteries
• ISSN: 0002-9610; 1879-1883
• DOI: 10.1016/S0002-9610(00)00278-6

Background: Hepatocyte membrane potential differences (PDs) may be altered by the preservation solutions used in liver transplantation. Such alterations could impact on the survival of the donor liver, extent of biochemical injury, and flux of important ionic compounds. The purpose of the present study was to document these outcomes in the presence of four different preservation solutions. Methods: Livers of adult male Sprague-Dawley rats (N = 3 to 4 per group) were impaled with intracellular microelectrodes prior to and at various time periods for 6 hours following complete hepatic resection. Just prior to resection, each liver was perfused with preservation solutions associated with high (normal saline [NS]), moderate (Euro-Collins [EC]), and low (University of Wisconsin solution [UW]) risks of reperfusion injury. Results: Baseline (in situ) PDs were similar in all groups (−37 ± 4 mV, mean ± SD). Ten minutes postresection, hepatic PDs were as follows: NS, −23.8 ± 3.5 mV; EC, −11.4 ± 0.4 mV; and UW, −8.7 ± 0.3 mV ( P