ROG, Repressor of GATA, Regulates the Expression of Cytokine Genes

• Published in: Immunity (Cambridge, Mass.) Mass.), 2000-03, Vol.12 (3), p.323-333
• Main Authors: Miaw, Shi-Chuen, Choi, Andrew, Yu, Elaine, Kishikawa, Hiroko, Ho, I-Cheng
• Format: Article
• Language: English
• Subjects: AIDS/HIV; Amino Acid Sequence; Animals; Base Sequence; Binding Sites; Chromosome Mapping; Cloning, Molecular; Cytokines - biosynthesis; Cytokines - genetics; DNA - metabolism; DNA, Complementary; DNA-Binding Proteins - antagonists & inhibitors; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; GATA-3 protein; GATA3 Transcription Factor; Gene Expression Regulation; Humans; Jurkat Cells; Kruppel-Like Transcription Factors; Lymphocytes - metabolism; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Precipitin Tests; Promyelocytic Leukemia Zinc Finger Protein; Protein Binding; Repressor Proteins - genetics; Repressor Proteins - metabolism; ROG protein; Th1 Cells - metabolism; Th2 Cells - metabolism; Trans-Activators - antagonists & inhibitors; Trans-Activators - genetics; Trans-Activators - metabolism; Transcription Factors - genetics; Transcriptional Activation; Tumor Cells, Cultured; Zinc Fingers
• ISSN: 1074-7613; 1097-4180
• DOI: 10.1016/S1074-7613(00)80185-5

GATA-3 is a T cell–specific transcription factor and is essential for the development of the T cell lineage. Recently, it was shown that the expression of GATA-3 is further induced in CD4 + helper T cells upon differentiation into type 2 but not type 1 effector cells. Here, we report the molecular cloning of a GATA-3 interacting protein, repressor of GATA (ROG). ROG is a lymphoid-specific gene and is rapidly induced in Th cells upon stimulation with anti-CD3. In in vitro assays, ROG represses the GATA-3-induced transactivation. Furthermore, overexpression of ROG in Th clones inhibits the production of Th cytokines. Taken together, our results suggest that ROG might play a critical role in regulating the differentiation and activation of Th cells.