Expression analysis of Escherichia coli lacZ reporter gene in transgenic mice

To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualiza...

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Bibliographic Details
Published in:Brain research. Brain research protocols 2000-04, Vol.5 (2), p.159-166
Main Authors: Takahashi, Eiki, Miyamoto, Norimasa, Kajiwara, Noriko, Furuya, Keiko, Yanai-Taniguchi, Keiko, Sugiyama, Fumihiro, Yagami, Ken-ichi
Format: Article
Language:English
Subjects:
Adrenal Glands - metabolism
Animals
beta-Galactosidase - metabolism
Brain - cytology
Brain - metabolism
Calcium Channels, P-Type - genetics
Calcium Channels, P-Type - metabolism
cis-Regulatory element
Escherichia coli - genetics
Gene Expression
Genes, Reporter - genetics
Lac Operon - genetics
lacZ
Mice
Mice, Inbred ICR
Mice, Transgenic - genetics
Mice, Transgenic - metabolism
Neurons - metabolism
Staining and Labeling
Tissue Distribution
Transcriptional regulation
Transgenic mice
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Summary:To define a gene expression mechanism, it is often advantageous to use a reporter gene and transgenic mouse. The lacZ reporter gene is particularly useful for studies of the cis-regulatory element for tissue-specific expression in transgenic mice because of the ease of the enzyme assay and visualization on sections. In this report, we describe our method for examining the cis-regulatory element in transgenic mice, including choice of the lacZ gene, generation of transgenic mice, and analysis of β-galactosidase activity. Themes: Cellular and molecular biology Topics: Staining, tracing, and imaging techniques
ISSN:1385-299X
DOI:10.1016/S1385-299X(00)00009-X