DNA vaccination against v- src oncogene-induced tumours in congenic chickens

DNA vaccination is particularly efficient for induction of cytotoxic T-lymphocyte (CTL) response. In our experiments, we used MHC( B) congenic chicken lines CB and CC (regressors and progressors of v- src-induced tumours, respectively) and a mutated, non-oncogenic v- src gene construct as the DNA va...

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Bibliographic Details
Published in:Vaccine 2001-08, Vol.19 (31), p.4526-4535
Main Authors: Plachý, Jiřı́, Hejnar, Jiřı́, Trtková, Kateřina, Trejbalová, Kateřina, Svoboda, Jan, Hála, Karel
Format: Article
Language:English
Subjects:
Adoptive Transfer - methods
Age Factors
Animals
Animals, Congenic
Avian Sarcoma Viruses - genetics
Avian Sarcoma Viruses - immunology
Biological and medical sciences
Cell Transformation, Viral
Chick Embryo
Chicken MHC( B)
Chickens
DNA vaccination
Dose-Response Relationship, Immunologic
Epidemiology. Vaccinations
Fundamental and applied biological sciences. Psychology
General aspects
Genes, src - genetics
Genes, src - immunology
Infectious diseases
Medical sciences
Microbiology
Oncogene Protein pp60(v-src) - immunology
Sarcoma, Avian - immunology
Sarcoma, Avian - prevention & control
v- src-specific immunity
v-src gene
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, DNA - therapeutic use
Viral Vaccines - genetics
Viral Vaccines - therapeutic use
Virology
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Summary:DNA vaccination is particularly efficient for induction of cytotoxic T-lymphocyte (CTL) response. In our experiments, we used MHC( B) congenic chicken lines CB and CC (regressors and progressors of v- src-induced tumours, respectively) and a mutated, non-oncogenic v- src gene construct as the DNA vaccine. A high degree of vaccine protection against oncogenic v- src challenge was achieved in the CB line chickens. CTL response was demonstrated in vitro and by adoptive transfer of immune cells to the syngeneic host and to the CC line chickens rendered tolerant to CB cells. In the CC line chickens we observed tumour growth retardation after a low-dose DNA vaccination administered to immature recipients while higher amounts of DNA vaccine in immunocompetent chickens exerted an enhancing effect.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(01)00181-5