Solution structure and dynamics of biomolecules from Raman optical activity

Raman optical activity (ROA) measures vibrational optical activity by means of a small difference in the intensity of Raman scattering from chiral molecules in right and left circularly polarized incident laser light. The ROA spectra of a wide range of biomolecules in aqueous solution can now be mea...

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Bibliographic Details
Published in:Progress in Biophysics and Molecular Biology 2000, Vol.73 (1), p.1-49
Main Authors: Barron, L.D., Hecht, L., Blanch, E.W., Bell, A.F.
Format: Article
Language:English
Subjects:
Animals
Biomolecular structure and dynamics
Carbohydrates
Carbohydrates - chemistry
Humans
Nucleic acids
Nucleic Acids - chemistry
Peptides - chemistry
Proteins
Proteins - chemistry
Raman optical activity
Spectrum Analysis, Raman - methods
Vibrational optical activity
Viruses
Viruses - chemistry
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Summary:Raman optical activity (ROA) measures vibrational optical activity by means of a small difference in the intensity of Raman scattering from chiral molecules in right and left circularly polarized incident laser light. The ROA spectra of a wide range of biomolecules in aqueous solution can now be measured routinely. Because of its sensitivity to the chiral elements of biomolecular structure, ROA provides new information about solution structure and dynamics complementary to that supplied by conventional spectroscopic techniques. This article provides a brief introduction to the theory and practice of ROA spectroscopy followed by a review of recent ROA results on polypeptides, proteins, carbohydrates, nucleic acids and viruses which illustrate how new insight into current problems of structure, folding and function may be obtained from ROA studies.
ISSN:0079-6107
1873-1732
DOI:10.1016/S0079-6107(99)00017-6