Nitration of manganese superoxide dismutase in cerebrospinal fluids is a marker for peroxynitrite-mediated oxidative stress in neurodegenerative diseases

Peroxynitrite can nitrate tyrosine residues of proteins. We examined nitrotyrosine‐containing proteins in cerebrospinal fluid of 66 patients with neurogenic disease by immunoblot analysis. Nitrated tyrosine residue–containing protein was observed in the cerebrospinal fluid and was concluded to be ma...

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Bibliographic Details
Published in:Annals of neurology 2000-04, Vol.47 (4), p.524-527
Main Authors: Aoyama, Koji, Matsubara, Kazuo, Fujikawa, Yasunori, Nagahiro, Yukio, Shimizu, Keiko, Umegae, Nobuyuki, Hayase, Nobumasa, Shiono, Hiroshi, Kobayashi, Shotai
Format: Article
Language:English
Subjects:
Adult
Aged
Alzheimer Disease - cerebrospinal fluid
Alzheimer Disease - enzymology
Antibodies
Biological and medical sciences
Biomarkers
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Female
Humans
Male
Medical sciences
Middle Aged
Neurology
Nitrates - metabolism
Nitrogen - metabolism
Oxidative Stress - physiology
Parkinson Disease - cerebrospinal fluid
Parkinson Disease - enzymology
Precipitin Tests
Superoxide Dismutase - cerebrospinal fluid
Tyrosine - analogs & derivatives
Tyrosine - analysis
Tyrosine - immunology
Tyrosine - metabolism
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Summary:Peroxynitrite can nitrate tyrosine residues of proteins. We examined nitrotyrosine‐containing proteins in cerebrospinal fluid of 66 patients with neurogenic disease by immunoblot analysis. Nitrated tyrosine residue–containing protein was observed in the cerebrospinal fluid and was concluded to be manganese superoxide dismutase (Mn‐SOD). The nitrated Mn‐SOD level was strikingly elevated in amyotrophic lateral sclerosis patients and was slightly increased in Alzheimer's and Parkinson's disease patients, whereas an elevated Mn‐SOD level was observed only in progressive supranuclear palsy group. Ann Neurol 2000;47:524–527.
ISSN:0364-5134
1531-8249
DOI:10.1002/1531-8249(200004)47:4<524::AID-ANA19>3.0.CO;2-5