Proposed phytic acid standard including a method for its analysis

A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous...

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Bibliographic Details
Published in:Journal of AOAC International 2001-07, Vol.84 (4), p.1125-1129
Main Authors: BROOKS, Stephen P. J, OBERLEAS, Donald, DAWSON, Brian A, BELONJE, Bartholomeus, LAMPI, Brian J
Format: Article
Language:English
Subjects:
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Magnetic Resonance Spectroscopy
Methods of analysis, processing and quality control, regulation, standards
Phosphorus - analysis
Phytic Acid - analysis
Phytic Acid - standards
Reference Standards
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Summary:A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous measurement with a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent in a microplate reader at 660 nm. The procedure can be performed in 3 h with as little as 50 mg sample.
ISSN:1060-3271
1944-7922
DOI:10.1093/jaoac/84.4.1125