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Proposed phytic acid standard including a method for its analysis

A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous...

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Published in:Journal of AOAC International 2001-07, Vol.84 (4), p.1125-1129
Main Authors: BROOKS, Stephen P. J, OBERLEAS, Donald, DAWSON, Brian A, BELONJE, Bartholomeus, LAMPI, Brian J
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container_issue 4
container_start_page 1125
container_title Journal of AOAC International
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creator BROOKS, Stephen P. J
OBERLEAS, Donald
DAWSON, Brian A
BELONJE, Bartholomeus
LAMPI, Brian J
description A method is described that accurately and rapidly quantifies the free and total phosphorous content of a commercially available, purified, phytic acid preparation. This allows its use as a standard for phytic acid determinations in foods. The method involves a wet ashing step followed by phosphorous measurement with a 1-amino-2-naphthol-4-sulfonic acid-molybdate reagent in a microplate reader at 660 nm. The procedure can be performed in 3 h with as little as 50 mg sample.
doi_str_mv 10.1093/jaoac/84.4.1125
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source Oxford University Press:Jisc Collections:OUP Read and Publish 2024-2025 (2024 collection) (Reading list)
subjects Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Magnetic Resonance Spectroscopy
Methods of analysis, processing and quality control, regulation, standards
Phosphorus - analysis
Phytic Acid - analysis
Phytic Acid - standards
Reference Standards
title Proposed phytic acid standard including a method for its analysis
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