Isolation of Orientia tsutsugamushi from Leptotrombidium fuji and Its Characterization

In our attempts to isolate Orientia tsutsugamushi from trombiculid mites, a strain was successfully isolated from Leptotrombidium fuji collected in Aichi Prefecture, Japan. This is the first case of isolation of O. tsutsugamushi from L. fuji. A phylogenetic analysis based on the base-sequence homolo...

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Bibliographic Details
Published in:MICROBIOLOGY and IMMUNOLOGY 2000, Vol.44(3), pp.201-204
Main Authors: Tamura, Akira, Makisaka, Yukie, Kadosaka, Teruki, Enatsu, Teruyuki, Okubo, Koji, Koyama, Shoichiro, Yu, Qiang, Urakami, Hiroshi
Format: Article
Language:English
Subjects:
Animals
Antigens, Bacterial - genetics
Bacteriology
Biological and medical sciences
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
Genes, Bacterial
Japan
Leptotrombidium fuji
Leptotrombidium pallidum
Microbiology
Molecular Sequence Data
Orientia tsutsugamushi
Orientia tsutsugamushi - classification
Orientia tsutsugamushi - genetics
Orientia tsutsugamushi - immunology
Orientia tsutsugamushi - isolation & purification
Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains
Phylogeny
Polymerase Chain Reaction
Rickettsia
Sequence Analysis, DNA
Trombiculidae - microbiology
Typing of Orientia tsutsugamushi
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Summary:In our attempts to isolate Orientia tsutsugamushi from trombiculid mites, a strain was successfully isolated from Leptotrombidium fuji collected in Aichi Prefecture, Japan. This is the first case of isolation of O. tsutsugamushi from L. fuji. A phylogenetic analysis based on the base-sequence homology of the 56-kDa type-specific antigen-gene indicated that the strain is a new type which is not closely related to any strains analyzed previously. Three strains isolated from Leptotrombidium pallidum harvested at the same area were identified as being closely related to the JP-2 type (subtype-2 of Karp type in Japan) by phylogenetic analysis.
ISSN:0385-5600
1348-0421
DOI:10.1111/j.1348-0421.2000.tb02484.x