Determination of circulating hemoglobin mass and related quantities by using capillary blood

• Published in: Medicine and science in sports and exercise 2000-05, Vol.32 (5), p.1024-1027
• Main Authors: HÜTLER, M, BENEKE, R, BÖNING, D
• Format: Article
• Language: English
• Subjects: Adult; Biological and medical sciences; Capillaries; Carboxyhemoglobin - analysis; Erythrocyte Volume; Fundamental and applied biological sciences. Psychology; Hematocrit; Hemoglobinometry - methods; Hemoglobins - analysis; Humans; Male; Plasma Volume; Reproducibility of Results; Space life sciences; Spirometry; Veins; Vertebrates: blood, hematopoietic organs, reticuloendothelial system
• ISSN: 0195-9131; 1530-0315
• DOI: 10.1097/00005768-200005000-00022

A standardized carbon monoxide (CO) rebreathing procedure with measurements of CO-hemoglobin, hemoglobin concentration ([Hb]), and hematocrit (Hct) enables to determine total Hb mass (Hb(tot)), blood, erythrocyte, and plasma volume (BV, EV, and PV). These calculations are normally based on venous blood samples. However, micromethods also allow determinations from capillary blood. The accuracy of using capillary blood for Hb(tot), BV, EV, and PV determination was evaluated in 42 men (age: 25.1 +/- 4.0 yr, body mass: 80.3 +/- 9.6 kg) by comparison of capillary and venous data. Capillary Hb(tot) (962 +/- 110 g) did not differ from venous values (959 +/- 106 g). Hb(tot) values were highly correlated (r = 0.987, P < 0.001, SEE 18 g). Also, capillary and venous BV, PV, and EV were highly correlated (0.94 < r < 0.98), but slightly different (-2.7 to 0.9%) because of higher capillary than venous [Hb] and Hct. Coefficients of variation of repeated Hb(tot), EV, PV, and BV measurements (3.0-5.2%) were similar in capillary and venous blood. Calculation of Hb(tot) using capillary blood is as accurate and reliable as using venous blood.