Generation of electrospray from a solution predeposited on optical fibers coiled with a platinum wire

This study examines the feasibility of generating electrospray directly from the tip of two optical fibers bound together with Teflon tape. This approach does not require a capillary and syringe pump. The electrospray source is simply constructed by coiling the two optical fibers with a platinum (Pt...

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Bibliographic Details
Published in:Journal of the American Society for Mass Spectrometry 2000-05, Vol.11 (5), p.464-467
Main Authors: Kuo, Chih-Pin, Yuan, Cheng-Hui, Shiea, Jentaie
Format: Article
Language:English
Subjects:
Analytical, structural and metabolic biochemistry
Biological and medical sciences
Capillary tubes
Coiling
Electric field effects
Electrospraying
Enzymes
Exact sciences and technology
Feasibility studies
Fiber Optic Technology
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Grooves
Instruments, apparatus, components and techniques common to several branches of physics and astronomy
Insulin
Insulin - analysis
Ions
Lysozyme
Mass spectra
Mass spectrometers and related techniques
Mass spectrometry
Mass Spectrometry - methods
Muramidase - analysis
Optical Fibers
Phosphodiesterase Inhibitors - analysis
Physics
Piperazines - analysis
Platinum
Polytetrafluoroethylene
Proteins
Purines
Sildenafil Citrate
Solutions
Spectroscopic analysis
Sulfones
Tablets
Ubiquitins - analysis
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Summary:This study examines the feasibility of generating electrospray directly from the tip of two optical fibers bound together with Teflon tape. This approach does not require a capillary and syringe pump. The electrospray source is simply constructed by coiling the two optical fibers with a platinum (Pt) wire. The optical fibers extend beyond the Pt coil for approximately 1 cm. The sample solution is predeposited on the Pt coil by a micropipette. As the high voltage required for electrospray is applied to the coil, the sample solution moves along the grooves between the two optical fibers. A stable electrospray is subsequently generated at the tip of the fibers. The mass spectra of insulin, lysozyme, and ubiquitin are exactly the same as those obtained by conventional electrospray using a capillary and syringe pump. Rapid determination of the active ingredient in a tablet by this technique is demonstrated.
ISSN:1044-0305
1879-1123
DOI:10.1016/S1044-0305(00)00111-2