PNA Interference Mapping Demonstrates Functional Domains in the Noncoding RNA Xist

The noncoding RNA Xist has been shown to be essential for X-chromosome inactivation and to coat the inactive X-chromosome (Xi). Thus, an important question in understanding the formation of Xi is whether the binding reaction of Xist is necessary for X-chromosome inactivation. In this article, we dem...

Full description

Saved in:
Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 2001-07, Vol.98 (16), p.9215-9220
Main Authors: Beletskii, Anton, Hong, Young-Kwon, Pehrson, John, Egholm, Michael, Strauss, William M.
Format: Article
Language:English
Subjects:
Actinomycin
Animals
Base Sequence
Biological Sciences
Body regions
Cell nucleus
Cellular differentiation
Diploidy
Female
Fibroblasts
Genes
Genetics
histone H2.A
Histones - metabolism
Male
Molecular Sequence Data
peptide nucleic acid interference mapping
Peptide Nucleic Acids - chemistry
Ribonucleic acid
RNA
RNA - chemistry
RNA - metabolism
RNA interference
RNA, Long Noncoding
RNA, Untranslated - chemistry
RNA, Untranslated - metabolism
Sex chromosomes
Transcription Factors - chemistry
Transcription Factors - metabolism
X Chromosome
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The noncoding RNA Xist has been shown to be essential for X-chromosome inactivation and to coat the inactive X-chromosome (Xi). Thus, an important question in understanding the formation of Xi is whether the binding reaction of Xist is necessary for X-chromosome inactivation. In this article, we demonstrate the failure of X-chromosome silencing if the association of Xist with the X-chromosome is inhibited. The chromatin-binding region was functionally mapped and evaluated by using an approach for studying noncoding RNA function in living cells that we call peptide nucleic acid (PNA) interference mapping. In the reported experiments, a single 19-bp antisense cell-permeating PNA targeted against a particular region of Xist RNA caused the disruption of the Xi. The association of the Xi with macro-histone H2A is also disturbed by PNA interference mapping.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.161173098