Spontaneous single-channel activity of neuronal TRP5 channel recombinantly expressed in HEK293 cells

Mammalian homologues of the Drosophila transient receptor potential ( trp) protein (TRP) form Ca 2+ permeable cation channels activated in response to stimulation of G-protein-coupled receptors. Establishing biophysical characteristics of basal TRP activity is of great importance in understanding mo...

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Bibliographic Details
Published in:Neuroscience letters 2000-05, Vol.285 (2), p.111-114
Main Authors: Yamada, Hisanobu, Wakamori, Minoru, Hara, Yuji, Takahashi, Yasuo, Konishi, Kazuhiko, Imoto, Keiji, Mori, Yasuo
Format: Article
Language:English
Subjects:
Biological and medical sciences
Calcium Channels - biosynthesis
Calcium Channels - genetics
Calcium Channels - physiology
Cation channel
Cation Transport Proteins
Cell Line
Cell membranes. Ionic channels. Membrane pores
Cell structures and functions
Embryo, Mammalian
Fundamental and applied biological sciences. Psychology
HEK293 cell
Humans
Kidney
Membrane Potentials - genetics
Molecular and cellular biology
Neurons - metabolism
Neurons - physiology
Patch clamp
Patch-Clamp Techniques
Receptor-activated channel
Recombinant Proteins - biosynthesis
Recombinant Proteins - metabolism
Single-channel recording
Transient receptor potential protein
TRPC Cation Channels
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Summary:Mammalian homologues of the Drosophila transient receptor potential ( trp) protein (TRP) form Ca 2+ permeable cation channels activated in response to stimulation of G-protein-coupled receptors. Establishing biophysical characteristics of basal TRP activity is of great importance in understanding modulatory processes, which underlie enhancement of TRP activity via receptor stimulation. We have examined spontaneous activity of the TRP5 channel recombinantly expressed in human embryonic kidney cells, using the conventional whole-cell mode of the patch-clamp technique in a low-Ca 2+ external solution. The unitary Na + conductance of the TRP5 channel was linear, being 47.6 pS. By contrast, the open probability of the TRP5 channel showed a voltage-dependent decrease below −50 mV. These biophysical properties are important hallmarks in distinguishing the TRP5 channel in native neuronal preparations, whose spontaneous activity may contribute to control of resting membrane potentials and generation of action potentials.
ISSN:0304-3940
1872-7972
DOI:10.1016/S0304-3940(00)01033-8