Live Oral Poliovirus Vaccines do not Contain Detectable Simian Virus 40 (SV40) DNA

Prior to 1962, poliovirus vaccines produced in rhesus monkey kidney cells were contaminated with SV40. Recent studies reporting the detection of SV40 in human tumours raised concern that SV40 may be oncogenic in humans. To provide further assurance that currently used poliovirus vaccines are not con...

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Bibliographic Details
Published in:Biologicals 2000-03, Vol.28 (1), p.1-4
Main Authors: Sierra-Honigmann, Ana, Krause, Philip R.
Format: Article
Language:English
Subjects:
Consumer Product Safety
DNA, Viral - analysis
Drug Contamination
Humans
Poliovirus
Poliovirus Vaccine, Oral
Simian virus 40
Simian virus 40 - genetics
Simian virus 40 - isolation & purification
USA
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Summary:Prior to 1962, poliovirus vaccines produced in rhesus monkey kidney cells were contaminated with SV40. Recent studies reporting the detection of SV40 in human tumours raised concern that SV40 may be oncogenic in humans. To provide further assurance that currently used poliovirus vaccines are not contaminated with SV40, we used the polymerase chain reaction (PCR) to search for SV40 DNA in live oral poliovirus vaccines manufactured in the United States between 1972 and 1996. SV40 DNA sequences were not found in any of the vaccine lots tested.
ISSN:1045-1056
1095-8320
DOI:10.1006/biol.1999.0233