Automated determination of reboxetine by high-performance liquid chromatography with column-switching and ultraviolet detection

• Published in: Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 2000-03, Vol.740 (1), p.135-140
• Main Authors: Härtter, Sebastian, Weigmann, Harald, Hiemke, Christoph
• Format: Article
• Language: English
• Subjects: Adrenergic Uptake Inhibitors - analysis; Adrenergic Uptake Inhibitors - blood; Automation; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Humans; Medical sciences; Morpholines - analysis; Morpholines - blood; Neuropharmacology; Pharmacology. Drug treatments; Psychoanaleptics: cns stimulant, antidepressant agent, nootropic agent, mood stabilizer..., (alzheimer disease); Psychology. Psychoanalysis. Psychiatry; Psychopharmacology; Quality Control; Reboxetine; Spectrophotometry, Ultraviolet
• ISSN: 0378-4347; 1387-2273
• DOI: 10.1016/S0378-4347(00)00040-2

A fully automated method including column-switching and isocratic high-performance liquid chromatography (HPLC) was developed for quantitative analysis of the new antidepressant reboxetine, a noradrenaline reuptake inhibitor. After serum injection into the HPLC system and on-line sample clean-up on a silica C 8 (10×4.0 mm I.D.) clean-up column with an eluent consisting of 2.5% acetonitrile in deionized water, the chromatographic separation was performed on an analytical column (Lichrospher CN; 250×4.6 mm I.D.) with an eluent of acetonitrile–aqueous potassium phosphate buffer (0.008 M, pH 6.4) (50:50). The UV detector was set at 273 or 226 nm. The limit of quantification was about 15 ng/ml at 273 nm and about 4 ng/ml at 226 nm. The day-to-day relative standard deviation ranged between 2.7 and 6.7% with recovery rates ≥90%. Linear regression analyses revealed correlation coefficients >0.998. The method can be applied to therapeutic drug monitoring of reboxetine as well as pharmacokinetic studies.