Analysis of glutathione by capillary electrophoresis based on sample stacking

Glutathione is a small peptide, which participates in cellular oxidation‐reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis...

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Bibliographic Details
Published in:Electrophoresis 2001-07, Vol.22 (11), p.2351-2354
Main Authors: Shihabi, Zakaria K., Hinsdale, Mark E., Cheng, Che Ping
Format: Article
Language:English
Subjects:
Animals
Buffers
Capillary electrophoresis
Dogs
Electrophoresis, Capillary - methods
Electrophoresis, Capillary - statistics & numerical data
Erythrocytes - chemistry
Glutathione
Glutathione - analysis
Glutathione - blood
Glutathione Disulfide - analysis
Glutathione Disulfide - blood
Hydrogen-Ion Concentration
Myocardium - chemistry
Sensitivity and Specificity
Stacking
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Summary:Glutathione is a small peptide, which participates in cellular oxidation‐reduction and detoxification. It is present in most biological tissues at different concentrations. The oxidized and reduced forms of the peptide were measured in erythrocytes and myocardial tissue by capillary electrophoresis based on stacking. After tissue homogenization or hemolysis of the red blood cells, the samples were deproteinized with acetonitrile and injected filling about 13% of the capillary volume. The electrophoresis was performed at 10 kV using a separation buffer of 250 mM borate, 50 mM Tris, pH 8.0. Sample stacking increased the sensitivity of detection by 10–20‐fold.
ISSN:0173-0835
1522-2683
DOI:10.1002/1522-2683(20017)22:11<2351::AID-ELPS2351>3.0.CO;2-C