Epidemiological typing of Bordetella pertussis isolates : Recommendations for a standard methodology

Pertussis is re-emerging in vaccinated populations, and to gain insight into the reasons for this development population-based studies are necessary. Unfortunately, various techniques are used to study Bordetella pertussis populations, hampering comparison between studies. A standard methodology for...

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Bibliographic Details
Published in:European journal of clinical microbiology & infectious diseases 2000-04, Vol.19 (3), p.174-181
Main Authors: MOOI, F. R, HALLANDER, H, WIRSING VON KONIG, C. H, HOET, B, GUISO, N
Format: Article
Language:English
Subjects:
Bacterial Outer Membrane Proteins - genetics
Bacterial Typing Techniques - standards
Base Sequence
Biological and medical sciences
Bordetella pertussis
Bordetella pertussis - classification
Bordetella pertussis - genetics
Bordetella pertussis - isolation & purification
DNA Fingerprinting - standards
DNA fingerprints
Electrophoresis, Gel, Pulsed-Field
Enzymes
Humans
Infectious diseases
Investigative techniques, diagnostic techniques (general aspects)
Laboratories
Medical sciences
Miscellaneous. Technology
Molecular Sequence Data
Monoclonal antibodies
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Pertussis Toxin
Polymorphism, Genetic
Population studies
Population-based studies
Recombinant Fusion Proteins - genetics
Reference Standards
Sequence Analysis, DNA
Serotyping - standards
Specimen Handling
Vaccines
Virulence Factors, Bordetella - genetics
Whooping cough
Whooping Cough - epidemiology
Whooping Cough - microbiology
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Summary:Pertussis is re-emerging in vaccinated populations, and to gain insight into the reasons for this development population-based studies are necessary. Unfortunately, various techniques are used to study Bordetella pertussis populations, hampering comparison between studies. A standard methodology for epidemiological typing of Bordetella pertussis isolates is proposed which is based on serotyping, pulsed-field gel electrophoresis and gene typing. Such a standard approach will allow comparisons between studies performed in different laboratories. Comparisons may reveal whether the epidemiological differences observed between countries are due for instance to different Bordetella pertussis populations or different vaccines used.
ISSN:0934-9723
1435-4373
DOI:10.1007/s100960050455