Modulation by nitric oxide (NO) of the intensity of non-quantum mediator secretion in neuromuscular junctions in rats

Experiments on rat diaphragm muscle showed that the nitric oxide (NO) donors sodium nitroprusside SNP) and S-nitroso-N-acetylpenicillamine (SNAP). as well as L-arginine. a substrate for NO synthesis. decreased the level of muscle fiber hyperpolarization (the H effect) after blockade of cholinoceptor...

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Bibliographic Details
Published in:Neuroscience and behavioral physiology 2001-07, Vol.31 (4), p.451-455
Main Authors: Mukhtarov, M R, Urazaev, A K, Nikolskii, E E, Vyskocil, F
Format: Article
Language:English
Subjects:
Acetylcholine - metabolism
Animals
Enzyme Inhibitors - pharmacology
Excitatory Postsynaptic Potentials - drug effects
Extracellular Space - physiology
In Vitro Techniques
Membrane Potentials - drug effects
Motor Neurons - physiology
Muscle, Skeletal - innervation
Muscle, Skeletal - physiology
Nerve Endings - physiology
Neuromuscular Junction - metabolism
Neuromuscular Junction - physiology
Neurotransmitter Agents - metabolism
Neurotransmitter Agents - physiology
NG-Nitroarginine Methyl Ester - pharmacology
Nitric Oxide - biosynthesis
Nitric Oxide - physiology
Nitric Oxide Donors - pharmacology
Nitric Oxide Synthase - antagonists & inhibitors
Nitric Oxide Synthase Type III
Rats
Synapses - drug effects
Synapses - physiology
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Summary:Experiments on rat diaphragm muscle showed that the nitric oxide (NO) donors sodium nitroprusside SNP) and S-nitroso-N-acetylpenicillamine (SNAP). as well as L-arginine. a substrate for NO synthesis. decreased the level of muscle fiber hyperpolarization (the H effect) after blockade of cholinoceptors on the postsynaptic membrane by d-tubocurarine in conditions of irreversible inhibition of acetylcholinesterase with armine. Conversely, disruptions to NO synthesis in muscle fibers by the NO synthase blocker NG-nitro-L-arginine methyl ester (L-NAME) led to increases in the H effect both in vitro and in vivo. Inactivated solutions of sodium nitroprusside and inactive forms of arginine and NAME (D-arginine. D-NAME) had no effect on the magnitude of the H effect, while hemoglobin, which efficiently binds NO molecules, blocked the inhibitory effects of sodium nitroprusside. SNAP, and L-arginine on the magnitude of the H effect. All these points provide evidence that NO can function as a modulator of non-quantum mediator release in the neuromuscular junctions of warm-blooded animals.
ISSN:0097-0549
1573-899X
DOI:10.1023/A:1010453031902