Cell Growth and By-Product Formation in a Pyruvate Kinase Mutant of E. coli

In this paper, we report on the analysis of acid formation in an E. coli pyk mutant. The results demonstrate that acid formation is insignificant for both the wild‐type and the mutant at low glucose concentrations. However, at relatively high glucose concentrations, acid formation remains very low f...

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Bibliographic Details
Published in:Biotechnology progress 2001-07, Vol.17 (4), p.624-628
Main Authors: Zhu, T., Phalakornkule, C., Koepsel, R. R., Domach, M. M., Ataai, M. M.
Format: Article
Language:English
Subjects:
Acids - metabolism
Bacillus subtilis - genetics
Bacillus subtilis - metabolism
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
carbon dioxide
Carbon Dioxide - metabolism
Cell Division - genetics
Escherichia coli
Escherichia coli - genetics
Escherichia coli - metabolism
Fundamental and applied biological sciences. Psychology
glucose
Glucose - metabolism
Mission oriented research
Mutation
Phosphoenolpyruvate - metabolism
Physiology and metabolism
Pyruvate Kinase - genetics
Pyruvate Kinase - metabolism
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Summary:In this paper, we report on the analysis of acid formation in an E. coli pyk mutant. The results demonstrate that acid formation is insignificant for both the wild‐type and the mutant at low glucose concentrations. However, at relatively high glucose concentrations, acid formation remains very low for the mutant but is significant for the wild‐type. This substantial reduction in acids is accompanied by an increase in CO2 production. Moreover, unlike the B. subtilispyk mutant, the E. coli pyk mutant did not show a substantial increase in the PEP pool.
ISSN:8756-7938
1520-6033
DOI:10.1021/bp0100575