Expression of erbB-3 protein in colorectal adenocarcinoma : correlation with poor survival

The family of erbB receptors includes four transmembrane glycoproteins with tyrosine kinase activity. These receptors are widely expressed in normal tissues, but they also have been implicated in the development of several human adenocarcinomas. c-erbB-3/HER-3 has been detected to a greater or lesse...

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Bibliographic Details
Published in:Journal of cancer research and clinical oncology 2000-04, Vol.126 (4), p.205-211
Main Authors: KAPITANOVIC, S, RADOSEVIC, S, SLADE, N, KAPITANOVIC, M, ANDELINOVIC, S, FERENCIC, Z, TAVASSOLI, M, SPAVENTI, S, PAVELIC, K, SPAVENTI, R
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - metabolism
Adenocarcinoma - mortality
Biological and medical sciences
Biomarkers, Tumor - biosynthesis
Biomarkers, Tumor - genetics
Biomarkers, Tumor - immunology
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - mortality
Gastroenterology. Liver. Pancreas. Abdomen
Gene Amplification
Humans
Medical sciences
Prognosis
Receptor, ErbB-3 - biosynthesis
Receptor, ErbB-3 - genetics
Receptor, ErbB-3 - immunology
Regression Analysis
Retrospective Studies
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Survival Analysis
Tumor Cells, Cultured
Tumors
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Summary:The family of erbB receptors includes four transmembrane glycoproteins with tyrosine kinase activity. These receptors are widely expressed in normal tissues, but they also have been implicated in the development of several human adenocarcinomas. c-erbB-3/HER-3 has been detected to a greater or lesser extent in many tissues from the digestive, urinary, reproductive and respiratory tracts. The overexpression of c-erbB-3/HER-3 protein has also been shown in 53%-88% of colorectal adenocarcinomas. In this study we investigated the expression of the c-erbB-3/ HER-3 gene product in colorectal tumour samples, and compared the results obtained with several clinicopathological parameters, including the survival of patients. Paraffin-embedded tissue sections were analysed immunohistochemically, using monoclonal antibody RTJ1 to human erbB-3 protein. Antibody RTJ1 specificity was confirmed by immunoprecipitation followed by Western blotting analysis. Amplification of the erbB-3 oncogene was tested by dot-blot hybridization. Adenocarcinomas of the colon were positive for erbB-3 protein in 78% of samples examined. Dot-blot analysis showed no amplification of the erbB-3 gene in colon adenocarcinomas. Statistical analysis showed that patients with tumours that could not be stained for erbB-3 protein survived significantly longer (P
ISSN:0171-5216
1432-1335
DOI:10.1007/s004320050034