Genomic DNA Released by Dying Cells Induces the Maturation of APCs

Mature APCs play a key role in the induction of Ag-specific immunity. This work examines whether genomic DNA released by dying cells provides a stimulus for APC maturation. Double-stranded but not single-stranded genomic DNA triggered APC to up-regulate expression of MHC class I/II and various costi...

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Bibliographic Details
Published in:The Journal of immunology (1950) 2001-09, Vol.167 (5), p.2602-2607
Main Authors: Ishii, Ken J, Suzuki, Koichi, Coban, Cevayir, Takeshita, Fumihiko, Itoh, Yasushi, Matoba, Hana, Kohn, Leonard D, Klinman, Dennis M
Format: Article
Language:English
Subjects:
Animals
Antigen-Presenting Cells - cytology
Antigen-Presenting Cells - drug effects
Antigen-Presenting Cells - immunology
Base Sequence
Cell Death - drug effects
Cell Death - immunology
Cell Differentiation
Cell Line
DNA - genetics
DNA - immunology
DNA - metabolism
DNA - pharmacology
DNA Fragmentation
DNA, Bacterial - genetics
DNA, Bacterial - immunology
DNA, Bacterial - pharmacology
Female
In Vitro Techniques
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
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Summary:Mature APCs play a key role in the induction of Ag-specific immunity. This work examines whether genomic DNA released by dying cells provides a stimulus for APC maturation. Double-stranded but not single-stranded genomic DNA triggered APC to up-regulate expression of MHC class I/II and various costimulatory molecules. Functionally, dsDNA enhanced APC function in vitro and improved primary cellular and humoral immune responses in vivo. These effects were dependent on the length and concentration of the dsDNA but were independent of nucleotide sequence. The maturation of APC induced by dsDNA may promote host survival by improving immune surveillance at sites of tissue injury/infection.
ISSN:0022-1767
1550-6606
DOI:10.4049/jimmunol.167.5.2602