A new species of Fusarium producer of galactose oxidase

Fifty‐two isolates of Fusarium species and one of Gibberella fujikuroi were tested for galactose oxidase (GO) production. Five Fusarium isolates contained GO activity in the culture filtrate: three F. graminearum and one each F. moniliforme f. sp. subglutinans and F. acuminatum. This is the first ti...

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Bibliographic Details
Published in:Journal of basic microbiology 2001-07, Vol.41 (3-4), p.143-148
Main Authors: Barbosa-Tessmann, Ione Parra, da Silva, Dioni Antunes, Peralta, Rosane Marina, Kemmelmeier, Carlos
Format: Article
Language:English
Subjects:
Culture Media
Fusarium
Fusarium - classification
Fusarium - enzymology
Galactose Oxidase - biosynthesis
Galactose Oxidase - isolation & purification
Gibberella fujikuroi
Time Factors
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Summary:Fifty‐two isolates of Fusarium species and one of Gibberella fujikuroi were tested for galactose oxidase (GO) production. Five Fusarium isolates contained GO activity in the culture filtrate: three F. graminearum and one each F. moniliforme f. sp. subglutinans and F. acuminatum. This is the first time F. acuminatum is reported to be a producer of GO enzyme. GO enzyme activity produced by isolates was assayed through a time course. Moreover, GO protein was partially purified from the most productive four isolates to show that the activity measured in the culture filtrates was due to the presence of GO protein.
ISSN:0233-111X
1521-4028
DOI:10.1002/1521-4028(200107)41:3/4<143::AID-JOBM143>3.0.CO;2-G