Molecular Authentication of Panax ginseng Species by RAPD Analysis and PCR-RFLP

In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginsen...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2001, Vol.24(8), pp.872-875
Main Authors: UM, Jae-Young, CHUNG, Hwan-Suck, KIM, Mi-Sun, NA, Ho-Jeong, KWON, Hyun-Jeong, KIM, Jeong-Joong, LEE, Kang-Min, LEE, Seung Jae, LIM, Jong Phil, DO, Keum-Rok, HWANG, Woo-Jun, LYU, Yeoung-Su, AN, Nyeon-Hyoung, KIM, Hyung-Min
Format: Article
Language:English
Subjects:
authentication
Biological and medical sciences
DNA, Plant - chemistry
DNA, Plant - genetics
General pharmacology
Medical sciences
Panax - chemistry
Panax - genetics
Panax species
PCR-RFLP
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
Plant Roots - chemistry
Polymorphism, Restriction Fragment Length
Random Amplified Polymorphic DNA Technique
RAPD
Reverse Transcriptase Polymerase Chain Reaction
RNA, Ribosomal - chemistry
RNA, Ribosomal - genetics
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Summary:In order to develop convenient and reproducible methods for the identification of ginseng drugs at a DNA level, randomly amplified polymorphic DNA (RAPD) and PCR-restriction fragment length polymorphism (PCR-RFLP) analyses were applied within Panax species. To authenticate Panax ginseng among ginseng populations, RAPD analysis was carried out using a 20 mer-random primer. The similarity coefficients among the DNA of ginseng plants analyzed were low, ranging from 0.197 to 0.491. In addition, by using PCR-RFLP analysis, very different fingerprints were obtained within Korean ginseng plants. These results suggest that these methods are able to authenticate the concerned Panax species. Broader application of this approach to authenticate other morphologically similar medicinal materials is rationalized.
ISSN:0918-6158
1347-5215
DOI:10.1248/bpb.24.872