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Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis
OBJECTIVE: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that has been implicated in cancer growth, tissue development, and wound repair. Its actions are dependent on activation by HGF activator (HGFA) and its binding to a specific HGF receptor (c-Met). We examined the role of HGF,...
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| Published in: | Journal of rheumatology 2001-08, Vol.28 (8), p.1772-1778 |
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| Main Authors: | , , , , , , , |
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| container_end_page | 1778 |
| container_issue | 8 |
| container_start_page | 1772 |
| container_title | Journal of rheumatology |
| container_volume | 28 |
| creator | NAGASHIMA, Masakazu HASEGAWA, Jun KATO, Ko YAMAZAKI, Junji NISHIGAI, Keiko ISHIWATA, Toshiyuki ASANO, Goro YOSHINO, Shinichi |
| description | OBJECTIVE: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that has been implicated in cancer growth, tissue
development, and wound repair. Its actions are dependent on activation by HGF activator (HGFA) and its binding to a specific
HGF receptor (c-Met). We examined the role of HGF, HGFA, and c-Met in synovial tissues in rheumatoid arthritis (RA) and osteoarthritis
(OA), and their localization and mRNA expression. METHODS: Immunohistochemical staining, Western blotting, RT-PCR, and in
situ hybridization (ISH) for HGF, HGFA, and c-Met were performed on synovial tissue specimens from 10 patients with RA and
4 with OA, and 2 healthy controls. RESULTS: Immunohistochemical staining revealed that HGFA and c-Met were strongly expressed
in fibroblasts, macrophages, endothelial cells, and synovial lining cells. HGF was expressed only faintly in macrophages and
fibroblasts, and not at all in the endothelial cells of RA and OA synovial tissue. HGFA was detected near 73 and 34 kDa on
Western blot analysis, corresponding to inactive and active HGFA, respectively. RT-PCR showed HGF, HGFA, and c-Met mRNA in
RA, OA, and control synovial tissue. ISH and immunohistochemistry revealed mRNA expression for HGF, HGFA, and c-Met in the
cell types mentioned above. CONCLUSION: HGFA, HGF, and c-Met mRNA are expressed in synovial tissue in RA and OA, and HGF is
activated by HGFA and binds to c-Met on endothelial cells, inducing angiogenesis. |
| format | article |
| fullrecord | <record><control><sourceid>proquest_pubme</sourceid><recordid>TN_cdi_proquest_miscellaneous_71105121</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>71105121</sourcerecordid><originalsourceid>FETCH-LOGICAL-h268t-5aeaf6ed3408088d1327f54a35de7a8f001ec81a993efda1df1d4e0bcf03348a3</originalsourceid><addsrcrecordid>eNpFkNtKAzEQhhdRbK2-guRGUehCstlD9lKKbYWKNwreLdPNpBvZQ02yXfr2Rq32Zgb--eafw0kwZnGeh1GWRKfBmHKWhCyN3kfBhbUflLI0TsV5MGIsoSLJxDgYlrgF15V7h2RjusFVREHpOkPulov5_ZT4SLygd54yUwKtJGX4jI7olth92-001MRpa3u035qpsG88qyUB4yqjfe2nq7MOu3_pMjhTUFu8OuRJ8DZ_fJ0tw9XL4mn2sAqrKBUuTABBpSh5TAUVQjIeZSqJgScSMxDKn4SlYJDnHJUEJhWTMdJ1qSjnsQA-CW5_fbem-_QruqLRtsS6hha73hYZYzRhEfPg9QHs1w3KYmt0A2Zf_L3KAzcHAGwJtTLQltoeuZim_r38OLHSm2rQBgvbQF17W14MwxCJQhQsyyL-BY6Hgco</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>71105121</pqid></control><display><type>article</type><title>Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis</title><source>Freely Accessible Journals</source><creator>NAGASHIMA, Masakazu ; HASEGAWA, Jun ; KATO, Ko ; YAMAZAKI, Junji ; NISHIGAI, Keiko ; ISHIWATA, Toshiyuki ; ASANO, Goro ; YOSHINO, Shinichi</creator><creatorcontrib>NAGASHIMA, Masakazu ; HASEGAWA, Jun ; KATO, Ko ; YAMAZAKI, Junji ; NISHIGAI, Keiko ; ISHIWATA, Toshiyuki ; ASANO, Goro ; YOSHINO, Shinichi</creatorcontrib><description>OBJECTIVE: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that has been implicated in cancer growth, tissue
development, and wound repair. Its actions are dependent on activation by HGF activator (HGFA) and its binding to a specific
HGF receptor (c-Met). We examined the role of HGF, HGFA, and c-Met in synovial tissues in rheumatoid arthritis (RA) and osteoarthritis
(OA), and their localization and mRNA expression. METHODS: Immunohistochemical staining, Western blotting, RT-PCR, and in
situ hybridization (ISH) for HGF, HGFA, and c-Met were performed on synovial tissue specimens from 10 patients with RA and
4 with OA, and 2 healthy controls. RESULTS: Immunohistochemical staining revealed that HGFA and c-Met were strongly expressed
in fibroblasts, macrophages, endothelial cells, and synovial lining cells. HGF was expressed only faintly in macrophages and
fibroblasts, and not at all in the endothelial cells of RA and OA synovial tissue. HGFA was detected near 73 and 34 kDa on
Western blot analysis, corresponding to inactive and active HGFA, respectively. RT-PCR showed HGF, HGFA, and c-Met mRNA in
RA, OA, and control synovial tissue. ISH and immunohistochemistry revealed mRNA expression for HGF, HGFA, and c-Met in the
cell types mentioned above. CONCLUSION: HGFA, HGF, and c-Met mRNA are expressed in synovial tissue in RA and OA, and HGF is
activated by HGFA and binds to c-Met on endothelial cells, inducing angiogenesis.</description><identifier>ISSN: 0315-162X</identifier><identifier>EISSN: 1499-2752</identifier><identifier>PMID: 11508578</identifier><identifier>CODEN: JRHUA9</identifier><language>eng</language><publisher>Toronto, ON: The Journal of Rheumatology</publisher><subject>Arthritis, Rheumatoid - metabolism ; Biological and medical sciences ; Blotting, Western ; Diseases of the osteoarticular system ; Hepatocyte Growth Factor - analysis ; Hepatocyte Growth Factor - genetics ; Humans ; Immunohistochemistry ; In Situ Hybridization ; Inflammatory joint diseases ; Medical sciences ; Neovascularization, Pathologic - metabolism ; Osteoarthritis - metabolism ; Proto-Oncogene Proteins c-met - analysis ; Proto-Oncogene Proteins c-met - genetics ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis ; Serine Endopeptidases - analysis ; Serine Endopeptidases - genetics ; Synovial Membrane - chemistry ; Synovial Membrane - physiology</subject><ispartof>Journal of rheumatology, 2001-08, Vol.28 (8), p.1772-1778</ispartof><rights>2002 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=14061643$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11508578$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>NAGASHIMA, Masakazu</creatorcontrib><creatorcontrib>HASEGAWA, Jun</creatorcontrib><creatorcontrib>KATO, Ko</creatorcontrib><creatorcontrib>YAMAZAKI, Junji</creatorcontrib><creatorcontrib>NISHIGAI, Keiko</creatorcontrib><creatorcontrib>ISHIWATA, Toshiyuki</creatorcontrib><creatorcontrib>ASANO, Goro</creatorcontrib><creatorcontrib>YOSHINO, Shinichi</creatorcontrib><title>Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis</title><title>Journal of rheumatology</title><addtitle>J Rheumatol</addtitle><description>OBJECTIVE: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that has been implicated in cancer growth, tissue
development, and wound repair. Its actions are dependent on activation by HGF activator (HGFA) and its binding to a specific
HGF receptor (c-Met). We examined the role of HGF, HGFA, and c-Met in synovial tissues in rheumatoid arthritis (RA) and osteoarthritis
(OA), and their localization and mRNA expression. METHODS: Immunohistochemical staining, Western blotting, RT-PCR, and in
situ hybridization (ISH) for HGF, HGFA, and c-Met were performed on synovial tissue specimens from 10 patients with RA and
4 with OA, and 2 healthy controls. RESULTS: Immunohistochemical staining revealed that HGFA and c-Met were strongly expressed
in fibroblasts, macrophages, endothelial cells, and synovial lining cells. HGF was expressed only faintly in macrophages and
fibroblasts, and not at all in the endothelial cells of RA and OA synovial tissue. HGFA was detected near 73 and 34 kDa on
Western blot analysis, corresponding to inactive and active HGFA, respectively. RT-PCR showed HGF, HGFA, and c-Met mRNA in
RA, OA, and control synovial tissue. ISH and immunohistochemistry revealed mRNA expression for HGF, HGFA, and c-Met in the
cell types mentioned above. CONCLUSION: HGFA, HGF, and c-Met mRNA are expressed in synovial tissue in RA and OA, and HGF is
activated by HGFA and binds to c-Met on endothelial cells, inducing angiogenesis.</description><subject>Arthritis, Rheumatoid - metabolism</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Diseases of the osteoarticular system</subject><subject>Hepatocyte Growth Factor - analysis</subject><subject>Hepatocyte Growth Factor - genetics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>In Situ Hybridization</subject><subject>Inflammatory joint diseases</subject><subject>Medical sciences</subject><subject>Neovascularization, Pathologic - metabolism</subject><subject>Osteoarthritis - metabolism</subject><subject>Proto-Oncogene Proteins c-met - analysis</subject><subject>Proto-Oncogene Proteins c-met - genetics</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><subject>Serine Endopeptidases - analysis</subject><subject>Serine Endopeptidases - genetics</subject><subject>Synovial Membrane - chemistry</subject><subject>Synovial Membrane - physiology</subject><issn>0315-162X</issn><issn>1499-2752</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNpFkNtKAzEQhhdRbK2-guRGUehCstlD9lKKbYWKNwreLdPNpBvZQ02yXfr2Rq32Zgb--eafw0kwZnGeh1GWRKfBmHKWhCyN3kfBhbUflLI0TsV5MGIsoSLJxDgYlrgF15V7h2RjusFVREHpOkPulov5_ZT4SLygd54yUwKtJGX4jI7olth92-001MRpa3u035qpsG88qyUB4yqjfe2nq7MOu3_pMjhTUFu8OuRJ8DZ_fJ0tw9XL4mn2sAqrKBUuTABBpSh5TAUVQjIeZSqJgScSMxDKn4SlYJDnHJUEJhWTMdJ1qSjnsQA-CW5_fbem-_QruqLRtsS6hha73hYZYzRhEfPg9QHs1w3KYmt0A2Zf_L3KAzcHAGwJtTLQltoeuZim_r38OLHSm2rQBgvbQF17W14MwxCJQhQsyyL-BY6Hgco</recordid><startdate>20010801</startdate><enddate>20010801</enddate><creator>NAGASHIMA, Masakazu</creator><creator>HASEGAWA, Jun</creator><creator>KATO, Ko</creator><creator>YAMAZAKI, Junji</creator><creator>NISHIGAI, Keiko</creator><creator>ISHIWATA, Toshiyuki</creator><creator>ASANO, Goro</creator><creator>YOSHINO, Shinichi</creator><general>The Journal of Rheumatology</general><general>Journal of Rheumatology Publishing</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20010801</creationdate><title>Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis</title><author>NAGASHIMA, Masakazu ; HASEGAWA, Jun ; KATO, Ko ; YAMAZAKI, Junji ; NISHIGAI, Keiko ; ISHIWATA, Toshiyuki ; ASANO, Goro ; YOSHINO, Shinichi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-5aeaf6ed3408088d1327f54a35de7a8f001ec81a993efda1df1d4e0bcf03348a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><topic>Arthritis, Rheumatoid - metabolism</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Diseases of the osteoarticular system</topic><topic>Hepatocyte Growth Factor - analysis</topic><topic>Hepatocyte Growth Factor - genetics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>In Situ Hybridization</topic><topic>Inflammatory joint diseases</topic><topic>Medical sciences</topic><topic>Neovascularization, Pathologic - metabolism</topic><topic>Osteoarthritis - metabolism</topic><topic>Proto-Oncogene Proteins c-met - analysis</topic><topic>Proto-Oncogene Proteins c-met - genetics</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><topic>Serine Endopeptidases - analysis</topic><topic>Serine Endopeptidases - genetics</topic><topic>Synovial Membrane - chemistry</topic><topic>Synovial Membrane - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NAGASHIMA, Masakazu</creatorcontrib><creatorcontrib>HASEGAWA, Jun</creatorcontrib><creatorcontrib>KATO, Ko</creatorcontrib><creatorcontrib>YAMAZAKI, Junji</creatorcontrib><creatorcontrib>NISHIGAI, Keiko</creatorcontrib><creatorcontrib>ISHIWATA, Toshiyuki</creatorcontrib><creatorcontrib>ASANO, Goro</creatorcontrib><creatorcontrib>YOSHINO, Shinichi</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of rheumatology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NAGASHIMA, Masakazu</au><au>HASEGAWA, Jun</au><au>KATO, Ko</au><au>YAMAZAKI, Junji</au><au>NISHIGAI, Keiko</au><au>ISHIWATA, Toshiyuki</au><au>ASANO, Goro</au><au>YOSHINO, Shinichi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis</atitle><jtitle>Journal of rheumatology</jtitle><addtitle>J Rheumatol</addtitle><date>2001-08-01</date><risdate>2001</risdate><volume>28</volume><issue>8</issue><spage>1772</spage><epage>1778</epage><pages>1772-1778</pages><issn>0315-162X</issn><eissn>1499-2752</eissn><coden>JRHUA9</coden><abstract>OBJECTIVE: Hepatocyte growth factor (HGF) is a multifunctional polypeptide that has been implicated in cancer growth, tissue
development, and wound repair. Its actions are dependent on activation by HGF activator (HGFA) and its binding to a specific
HGF receptor (c-Met). We examined the role of HGF, HGFA, and c-Met in synovial tissues in rheumatoid arthritis (RA) and osteoarthritis
(OA), and their localization and mRNA expression. METHODS: Immunohistochemical staining, Western blotting, RT-PCR, and in
situ hybridization (ISH) for HGF, HGFA, and c-Met were performed on synovial tissue specimens from 10 patients with RA and
4 with OA, and 2 healthy controls. RESULTS: Immunohistochemical staining revealed that HGFA and c-Met were strongly expressed
in fibroblasts, macrophages, endothelial cells, and synovial lining cells. HGF was expressed only faintly in macrophages and
fibroblasts, and not at all in the endothelial cells of RA and OA synovial tissue. HGFA was detected near 73 and 34 kDa on
Western blot analysis, corresponding to inactive and active HGFA, respectively. RT-PCR showed HGF, HGFA, and c-Met mRNA in
RA, OA, and control synovial tissue. ISH and immunohistochemistry revealed mRNA expression for HGF, HGFA, and c-Met in the
cell types mentioned above. CONCLUSION: HGFA, HGF, and c-Met mRNA are expressed in synovial tissue in RA and OA, and HGF is
activated by HGFA and binds to c-Met on endothelial cells, inducing angiogenesis.</abstract><cop>Toronto, ON</cop><pub>The Journal of Rheumatology</pub><pmid>11508578</pmid><tpages>7</tpages></addata></record> |
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| source | Freely Accessible Journals |
| subjects | Arthritis, Rheumatoid - metabolism Biological and medical sciences Blotting, Western Diseases of the osteoarticular system Hepatocyte Growth Factor - analysis Hepatocyte Growth Factor - genetics Humans Immunohistochemistry In Situ Hybridization Inflammatory joint diseases Medical sciences Neovascularization, Pathologic - metabolism Osteoarthritis - metabolism Proto-Oncogene Proteins c-met - analysis Proto-Oncogene Proteins c-met - genetics Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis Serine Endopeptidases - analysis Serine Endopeptidases - genetics Synovial Membrane - chemistry Synovial Membrane - physiology |
| title | Hepatocyte growth factor (HGF), HGF activator, and c-Met in synovial tissues in rheumatoid arthritis and osteoarthritis |
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