Negative regulation of the bolA1p of Escherichia coli K-12 by the transcription factor OmpR for osmolarity response genes

Abstract To test whether OmpR is involved in regulation of the bolA1p, we investigated possible effects of ompR mutation on transcription from bolA1p. In vivo, bolA1p was found to be repressed by OmpR. Furthermore in vitro, the phospho-OmpR was found to bind to the OmpR binding region of bolA1p and...

Full description

Saved in:
Bibliographic Details
Published in:FEMS microbiology letters 2000-05, Vol.186 (2), p.257-262
Main Authors: Yamamoto, Kaneyoshi, Nagura, Rie, Tanabe, Hiroyuki, Fujita, Nobuyuki, Ishihama, Akira, Utsumi, Ryutaro
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Base Sequence
Binding Sites
bolA
Consensus Sequence
Down-regulation
E coli
Escherichia coli
Escherichia coli - genetics
Gene expression
Gene Expression Regulation, Bacterial
Genes, Regulator
In vivo methods and tests
Kinetics
Microbiology
Mutation
OmpR
Osmolar Concentration
Osmolarity
Promoter Regions, Genetic
Stationary phase
Trans-Activators - genetics
Trans-Activators - metabolism
Transcription, Genetic
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract To test whether OmpR is involved in regulation of the bolA1p, we investigated possible effects of ompR mutation on transcription from bolA1p. In vivo, bolA1p was found to be repressed by OmpR. Furthermore in vitro, the phospho-OmpR was found to bind to the OmpR binding region of bolA1p and repress the transcription by EσS or EσD. These results suggest that the phosphorylated form of OmpR is a negative regulator for the transcription of the bolA1p promoter.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.2000.tb09114.x